食品科学 ›› 2017, Vol. 38 ›› Issue (10): 24-30.doi: 10.7506/spkx1002-6630-201710005

• 生物工程 • 上一篇    下一篇

植物乳杆菌YM-2菌株胞外多糖生物合成工艺优化

司天昭,柳陈坚,秦晓萌,李晓然,罗义勇,杨 恩   

  1. 昆明理工大学生命科学与技术学院,云南 昆明 650500
  • 出版日期:2017-05-25 发布日期:2017-05-23
  • 基金资助:
    国家自然科学基金地区科学基金项目(31560447)

Optimization of Biosynthesis Conditions for the Production of Exopolysaccharides by Lactobacillus plantarum YM-2

SI Tianzhao, LIU Chenjian, QIN Xiaomeng, LI Xiaoran, LUO Yiyong, YANG En   

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
  • Online:2017-05-25 Published:2017-05-23

摘要: 对从云南传统发酵豆豉分离得到的植物乳杆菌YM-2(Lactobacillus plantarum YM-2)菌株胞外多糖 (exopolysaccharide,EPS)生物合成条件进行优化。首先对培养基成分中的碳源、氮源种类进行筛选;然后利用 单因素试验分析碳源含量、氮源含量、培养时间以及培养温度对EPS产量的影响;最后采用Box-Behnken法进行 四因素三水平响应面分析以确定其最优工艺条件。结果表明,植物乳杆菌YM-2菌株生物合成EPS最佳条件为碳源 (葡萄糖)含量30 g/L、氮源(酵母粉)含量30 g/L、培养时间30.05 h、培养温度36.36 ℃,在此工艺条件下,植物 乳杆菌YM-2 EPS产量为257.362 mg/L。

关键词: 植物乳杆菌, 胞外多糖, 条件优化, 响应面法

Abstract: The fermentation conditions for the production of exopolysaccharides (EPS) by Lactobacillus plantarum YM-2, isolated from traditional fermented tempeh in Yunnan province, were optimized. Firstly, the carbon and nitrogen sources in the medium were screened. Secondly, the effects of carbon source concentration, nitrogen source concentration, culture time and culture temperature on EPS yield were studied through one-factor-at-a-time analysis. Finally, response surface methodology (RSM) based on a four-variable, three-level Box-Behnken design was used to determine the optimal culture conditions as follows: carbon source concentration, 30 g/L; nitrogen source concentration, 30 g/L; culture time, 30.05 h; and culture temperature, 36.36 ℃. Under these conditions, the yield of EPS was 257.362 mg/L.

Key words: Lactobacillus plantarum, exopolysaccharides (EPS), optimization, response surface methodology (RSM)

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