食品科学 ›› 2017, Vol. 38 ›› Issue (17): 14-19.doi: 10.7506/spkx1002-6630-201717003

• 基础研究 • 上一篇    下一篇

γ射线联合肉桂精油对腐败希瓦氏菌的抑制机制

吕 飞,徐 静,魏倩倩,高 飞,周绪霞,丁玉庭,刘 璘*   

  1. 浙江工业大学食品工程与质量控制研究所,浙江 杭州 310014
  • 出版日期:2017-09-15 发布日期:2017-09-12
  • 基金资助:
    国家自然科学基金青年科学基金项目(31301579)

Antimicrobial Mechanism of Combination of γ Irradiation and Cinnamon Essential Oil against Shewanella putrefaciens

Lü Fei, XU Jing, WEI Qianqian, GAO Fei, ZHOU Xuxia, DINY Yuting, LIU Lin*   

  1. Food Engineering and Quality Control Institution, Zhejiang University of Technology, Hangzhou 310014, China
  • Online:2017-09-15 Published:2017-09-12

摘要: 研究γ射线辐照和肉桂精油联合对腐败希瓦氏菌(Shewanella putrefaciens)的抑制效果,分析联合作用对S. putrefaciens的抑制机制。结果表明:辐照D10(菌落总数降低到原始数量的10%所需要的辐照剂量)随着肉桂精油质量浓度的增加而减少,S. putrefaciens对辐照的相对敏感度随肉桂精油质量浓度的增加而增加。辐照和肉桂精油联合对S. putrefaciens具有较好的抑制作用,其对S. putrefaciens细胞形态、胞内外ATP、胞内pH值、膜蛋白以及膜脂肪酸等均具有显著破坏作用。联合作用处理S. putrefaciens的膜蛋白40 kD亚基减少,18 kD亚基增加,且使S. putrefaciens主要膜脂肪酸C14∶0、C16∶0、C16∶1、C17∶1、C18∶1显著减少(P<0.05),其机理可能在于γ射线辐照增加了肉桂精油对S. putrefaciens细胞膜的破坏,从而导致其死亡。

关键词: 辐照, 肉桂精油, 腐败希瓦氏菌, 联合作用, 抑菌机理

Abstract: The combined antibacterial effect of γ irradiation and cinnamon essential oil (CEO) against Shewanella putrefaciens was evaluated and the underlying mechanism was investigated. The irradiation dose required to reduce total colony number to 10% of the initial level (D10) was reduced and the relative irradiation sensitivity was increased with increased concentration of CEO. The combination of γ irradiation and CEO was more effective in inhibiting the growth of S. putrefaciens than each alone. The combination treatment exhibited an obvious negative effect on cell morphology, intracellular ATP and pH, membrane proteins and fatty acids in S. putrefaciens. The combination resulted in a reduction of 40 kD subunits and an increase of 18 kD subunits in membrane proteins, as well as a significant reduction of C14:0, C16:0, C16:1, C17:1, and C18:1 in membrane lipids (P < 0.05). The mechanism for the synergism was due to the potential of γ irradiation to increase the destructive effect of CEO on the cell membrane of S. putrefaciens thus causing its death.

Key words: irradiation, cinnamon essential oil, Shewanella putrefaciens, synergistic effect, antimicrobial mechanism

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