金银花叶黄酮对衰老模型小鼠的体内抗氧化作用

doi:10.7506/spkx1002-6630-201719027

食品科学 ›› 2017, Vol. 38 ›› Issue (19): 171-176.doi: 10.7506/spkx1002-6630-201719027

金银花叶黄酮对衰老模型小鼠的体内抗氧化作用

罗磊，张冰洁，关宁宁，马丽苹，朱文学

河南科技大学食品与生物工程学院，河南省农产品干燥装备工程技术研究中心，河南洛阳 471023

出版日期:2017-10-15 发布日期:2017-09-29
基金资助:
国家自然科学基金联合基金项目（U1304330）；河南省高校科技创新团队计划项目（17IRTSTHN016）

In Vivo Antioxidant Activity of Honeysuckle Leaf Flavonoids in Aging Mice

LUO Lei, ZHANG Bingjie, GUAN Ningning, MA Liping, ZHU Wenxue

Henan Agricultural Products Drying Equipment Engineering Technology Research Center, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China

Online:2017-10-15 Published:2017-09-29

摘要/Abstract

摘要： 目的：研究金银花叶黄酮对衰老小鼠体内抗氧化系统的调节作用。方法：采用颈背部皮下注射D-半乳糖法构建衰老小鼠模型，用不同剂量（低、中、高剂量分别为50、100、200 mg/（kg?d））的金银花叶黄酮灌胃小鼠，观察小鼠体质量变化，并计算肝脏、脑、肾脏、脾脏及心脏指数，测定小鼠血清及肝脏、脑、肾脏及心脏组织中总抗氧化能力（total antioxidant capacity，T-AOC）、超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、过氧化氢酶（catalase，CAT）活力及丙二醛（malondialdehyde， MAD）、谷胱甘肽（glutathione，GSH）含量。结果：与空白组相比，模型组小鼠体质量和脏器指数显著下降，血清和各脏器组织中的T-AOC、SOD、GSH-Px、CAT活力及GSH含量均有所降低，MDA含量升高，且大部分差异显著（P＜0.05），说明衰老小鼠模型构建成功。与模型组相比，金银花叶黄酮各剂量组小鼠体质量和脏器指数均有所升高，血清和脏器中的T-AOC、SOD、GSH-Px、CAT活力及GSH含量均有不同程度的回升，MDA含量降低，高剂量组效果最显著。结论：金银花叶黄酮能有效提高衰老小鼠的抗氧化能力，其抗氧化机制可能与提高机体抗氧化酶活性和还原性保护物质含量、降低脂质过氧化物含量有关。

关键词: 金银花叶, 黄酮, 衰老模型小鼠, D-半乳糖, 抗氧化能力

Abstract: Objective: To study the effect of honeysuckle leaf flavonoids on the antioxidant defense system in various tissues of aging mice. Methods: An aging mouse model was established by subcutaneous injection of D-galactose in the neck and back, and the aging mice were gavaged with different doses (50, 100 and 200 mg/(kg·d)) of honeysuckle leaf flavonoids. Changes in body weight were observed over the 30-day administration period, and liver, brain, kidney, spleen and heart indexes and the contents of malondialdehyde (MDA) and glutathione (GSH) and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in the liver, brain, kidney, heart and serum of mice were assessed. Results: Compared with the control group, the body weight and organ indexes of mice in the model group decreased significantly, T-AOC, SOD, GSH-Px and CAT activities and GSH content in the serum and organs decreased, and MDA content increased, with significant differences being observed for most of these parameters (P < 0.05), indicating successful construction of aging mice model. Compared with the model group, body weight, organ indexes and T-AOC, SOD, GSH-Px and CAT activities as well as GSH content in the serum and organ tissues were improved for all dose groups; on the other hand, MDA content was decreased. The most significant effect was obtained at the high dose. Conclusion: Honeysuckle leaf flavonoids can effectively improve the antioxidant defense capacity of aging mice through a mechanism related to the increased activities of antioxidant enzymes and the reduced contents of protective substances and lipid peroxide.

Key words: honeysuckle leaf, flavonoids, aging mice, D-galactose, antioxidant capacity

中图分类号:

TS201.4

罗磊，张冰洁，关宁宁，马丽苹，朱文学. 金银花叶黄酮对衰老模型小鼠的体内抗氧化作用[J]. 食品科学, 2017, 38(19): 171-176.

LUO Lei, ZHANG Bingjie, GUAN Ningning, MA Liping, ZHU Wenxue. In Vivo Antioxidant Activity of Honeysuckle Leaf Flavonoids in Aging Mice[J]. FOOD SCIENCE, 2017, 38(19): 171-176.

[1]	王凤，肖楚翔，刘淑珍，王凤舞. 榴莲核黄酮的提取及其对秀丽隐杆线虫氧化和衰老的影响[J]. 食品科学, 2021, 42(9): 123-129.
[2]	贺文杰，吴彬彬，胥伟，王宏勋，易阳，雷飞飞. 热处理中芡实黄酮对肌原纤维蛋白结构的影响[J]. 食品科学, 2021, 42(6): 46-52.
[3]	周浓，余志良，李承勇. 玉米醇溶蛋白-番石榴黄酮复合纳米颗粒的制备及其抗氧化活性[J]. 食品科学, 2021, 42(3): 186-193.
[4]	陈林玉，宋乐园，王云雨，卢梦如，顿彩云，杨青华，毕跃峰. 红小米化学成分与营养成分分析[J]. 食品科学, 2021, 42(18): 218-224.
[5]	李思宁，唐善虎，任然. 动物双歧杆菌、植物乳杆菌与传统发酵剂共培养对发酵乳抗氧化特性的影响[J]. 食品科学, 2021, 42(18): 127-134.
[6]	阎然，傅茂润，陈蕾蕾，李有媛，徐敏慧，何志平，周庆新. 解淀粉芽孢杆菌NCPSJ7对采后脐橙绿霉病的防治作用及机制[J]. 食品科学, 2021, 42(17): 193-200.
[7]	丁羽萱，王尧，姚羿安，李皖梅，王冕，王沛，顾振新，杨润强. 外源γ-氨基丁酸对发芽大豆酚类物质富集及抗氧化能力的影响[J]. 食品科学, 2021, 42(13): 72-78.
[8]	高华武，王艳，周鹏，叶树，宋航，汪光云，蔡标. 金雀异黄酮通过调控Ca2＋-CaMKIV通路对Aβ25-35诱导海马神经元损伤的保护作用[J]. 食品科学, 2021, 42(13): 121-126.
[9]	沙玉欢，毛晓英，吴庆智，张建，程卫东. 核桃分心木黄酮物质的组分及其抗氧化性分析[J]. 食品科学, 2021, 42(12): 91-98.
[10]	窦露，刘畅，巴吉木色，杨致昊，苏琳，赵丽华，田建军，郭月英，靳烨. 日粮添加乳酸菌对苏尼特羊生长、肉品质、风味物质和抗氧化能力的影响[J]. 食品科学, 2021, 42(1): 25-32.
[11]	刘子微，朱建津，陈巍文，李浙烽. 阿魏酸类似物对强的松诱导的小鼠氧化应激的影响[J]. 食品科学, 2021, 42(1): 163-171.
[12]	张晓寒，赵江，韩英，刁翠茹，王浩. 根皮素对秀丽隐杆线虫寿命的影响[J]. 食品科学, 2021, 42(1): 187-196.
[13]	徐冉冉，袁洋，赵玉梅，曹建康. 低温气调贮藏对‘突尼斯’软籽石榴贮后货架期品质的影响[J]. 食品科学, 2020, 41(9): 153-160.
[14]	郝教敏，杨文平，靳明凯，赵芳芳，王凯，杨珍平，高志强. 黑麦多酚提取物对猪肉丸冷藏期间氧化稳定性和品质的影响[J]. 食品科学, 2020, 41(9): 175-181.
[15]	叶韬，陈志娜，叶倩文，刘慧乾，王云，陆剑锋. 不同杀菌方式对即食豆干大豆异黄酮及品质特性的影响[J]. 食品科学, 2020, 41(7): 124-130.

金银花叶黄酮对衰老模型小鼠的体内抗氧化作用

In Vivo Antioxidant Activity of Honeysuckle Leaf Flavonoids in Aging Mice

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价