食品科学 ›› 2017, Vol. 38 ›› Issue (2): 98-103.doi: 10.7506/spkx1002-6630-201702016

• 生物工程 • 上一篇    下一篇

发酵鲜湿米粉的优良菌种选育及品质研究

马 霞,李路遥,何 艳,程朝辉,于海燕   

  1. 1.上海应用技术大学香料香精技术与工程学院,上海 201418;2.上海绿晟实业有限公司,上海 202156
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    上海市联盟技术项目(LM201443)

Strain Screening and Quality Evaluation of Fermented Fresh Rice Noodles

MA Xia, LI Luyao, HE Yan, CHENG Zhaohui, YU Haiyan   

  1. 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; 2. Shanghai Lvsheng Industry Co. Ltd., Shanghai 202156, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 从大米发酵液中分离得到一株优势菌株,命名为S2219。经形态学特征、生理生化特性及16S rDNA基因序列分析鉴定其为乳酸乳球菌(Lactococcus lactis),进而比较纯菌种发酵、自然发酵及不发酵米粉的蛋白质、脂肪、淀粉含量、质构及感官品质。结果显示,纯菌种发酵米粉的蛋白质和脂肪质量分数均低于自然发酵和不发酵米粉,但其直链淀粉质量分数高于自然发酵和不发酵米粉;质构感官方面,纯菌种发酵明显改善了米粉的咀嚼性、弹性和回复性。米粉制备的最佳发酵方式是纯菌种发酵,其获得的米粉具有柔韧劲道、口感滑润的特点。

关键词: 乳酸乳球菌, 分离, 米粉, 蛋白质, 脂肪, 淀粉, 质构

Abstract: A dominant bacterial strain named S2219 was isolated from naturally fermented rice. This strain was identified as Lactococcus lactis based on its morphological, physiological and biochemical characteristics and 16S rDNA sequences. The protein, fat and starch contents, texture properties and sensory quality attributes of unfermented fresh rice noodles, naturally fermented fresh rice noodles and fresh rice noodles fermented by the strain S2219 were measured and compared. Our results indicated that the pure culture fermented rice noodles were lower in protein and fat but higher in amylose compared with the naturally fermented and unfermented samples. Moreover, the pure culture fermented rice noodles had significantly improved texture properties such as chewiness, springiness and resilience as well as a smoother taste than traditional fermented rice noodles. Accordingly, pure culture fermentation was the best choice for the production of fermented rice noodles.

Key words: Lactococcus lactis, isolation, rice noodles, protein, fat, starch, texture

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