食品科学 ›› 2017, Vol. 38 ›› Issue (23): 178-183.doi: 10.7506/spkx1002-6630-201723028

• 营养卫生 • 上一篇    下一篇

青藏高原牦牛酸奶中具有抗氧化活性乳酸菌的体内外益生特性

陈 明,柯文灿,张 娟,唐 京,王丽娜,丁武蓉*   

  1. 兰州大学生命科学学院,草地农业生态系统国家重点实验室,甘肃 兰州 730000
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家自然科学基金面上项目(31272486);兰州市人才创新创业项目(2016-RC-55);甘肃省中小企业创新基金项目(1608JCCA058)

Probiotic Properties in Vitro and in Vivo of Antioxidative Lactic Acid Bacteria from Yak Yogurt in Tibetan Plateau

CHEN Ming, KE Wencan, ZHANG Juan, TANG Jing, WANG Lina, DING Wurong*   

  1. State Key Laboratory of Grassland and Agro-Ecosystems, School of Life Science, Lanzhou University, Lanzhou 730000, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 目的:筛选出性状优良的具有抗氧化性的益生乳酸菌并研究其对D-半乳糖致衰老模型大鼠的抗氧化作用。 方法:以从青藏高原牦牛酸奶中初步筛选的3 株具高抗氧化活性乳酸菌为研究对象,通过耐受模拟胃肠道、耐胆盐 和疏水能力实验评价3 株乳酸菌的体外益生特性,并将体外益生特性优良的菌株饲喂D-半乳糖致衰老模型大鼠。通 过检测各处理组大鼠的脏器指数,肝脏、脑组织和血清中的谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)、 总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力、总抗氧化能力(total antioxidant capacity, T-AOC)以及丙二醛(malondialdehyde,MDA)含量来评价乳酸菌的体内抗氧化益生特性。结果:植物乳杆菌 (Lactobacillus plantarum)XM5具有较强的耐模拟胃肠液和耐胆盐能力,同时具有较强的疏水能力。植物乳杆菌 XM5的摄入能有效缓解D-半乳糖对大鼠脑组织的氧化损伤;使衰老模型大鼠肝脏中的GSH-Px活力,血清中的GSHPx 、T-SOD活力和T-AOC显著提高(P<0.05);同时使肝脏、脑组织和血清中的MDA含量显著降低(P<0.05)。 结论:植物乳杆菌XM5是一株性能优良的抗氧化益生乳酸菌,具有潜在的应用价值。

关键词: 青藏高原, 牦牛酸奶, 抗氧化, 乳酸菌, 益生特性

Abstract: Objective: To screen for lactic acid bacteria with excellent probiotic and antioxidant properties and to study their anti-aging effect on D-galactose-induced aging mice. Methods: Three strains of lactic acid bacteria with high antioxidant activity were isolated from yak yogurt in the Tibetan Plateau, and their probiotic properties in vitro were evaluated in terms of tolerance to simulated gastrointestinal fluid and bile salts and hydrophobic ability. The best strain was further selected and fed to the aging model mice. The probiotic properties in vivo were evaluated by organ indexes, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) activities, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content in brain tissue and serum of rats from each treatment group. Results: Lactobacillus plantarum XM5 had strong resistance to simulated gastrointestinal fluid and bile salt, and also had high hydrophobic ability. Intake of L. plantarum XM5 could effectively alleviate the oxidative damage in rat brain induced by D-galactose, significantly increase GSH-Px activity in liver, GSH-Px and T-SOD activities and T-AOC in serum and significantly reduce MDA content in liver, brain tissue and serum (P < 0.05). Conclusion: L. plantarum XM5 has great potential for application due to its excellent antioxidant properties.

Key words: Tibetan Plateau, yak yogurt, antioxidant, lactic acid bacteria, probiotic properties

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