• 工艺技术 •

纤维素载体固定化β-葡萄糖苷酶及其在大豆异黄酮水解中的应用

1. （1.南京财经大学食品科学与工程学院，江苏省现代粮食流通与安全协同创新中心，江苏?南京 210023；2.江南大学食品学院，江苏?无锡 214122）
• 出版日期:2017-12-25 发布日期:2017-12-07
• 基金资助:
江苏省高校自然科学研究面上项目（15KJB550006）；“十三五”国家重点研发计划重点专项（2016YFD0400201-1）； 江苏省产学研前瞻性联合研究项目（BY2016009-01）；江苏高校优势学科建设工程资助项目（PAPD）

Immobilization of β-Glucosidase onto Cellulose Particles for Application in Hydrolysis of Soybean Isoflavones

YAO Yijun, WANG Lifeng, JU Xingrong

1. (1. Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
• Online:2017-12-25 Published:2017-12-07

Abstract: This study was conducted to evaluate the feasibility of conversion of isoflavones in soymilk using cellulose-immobilized β-glucosidase. β-glucosidase was immobilized onto cellulose particles with a loading capacity of over 40 mg of enzyme/40 cm3 carrier. The immobilized enzyme exhibited a significant efficiency for the conversion of 4-nitrophenyl-β-D-glucuronide (p-NPG) to p-nitrophenol. The optimal temperature for the enzyme was 50 ℃ and the Michaelis constant (Km) value was determined to be (1.38 ± 0.20) mmol/L. All isoflavones in black soymilk were completely converted to deglycosylated derivatives within 30 min using 40 cm3 of the immobilized enzyme as analyzed by high-performance liquid chromatography (HPLC). Experimental evaluation of the stability showed that immobilized β-glucosidase retained 60% of its original activity after 15 times repeated use and after 15 days of storage.