食品科学 ›› 2017, Vol. 38 ›› Issue (24): 183-188.doi: 10.7506/spkx1002-6630-201724029

• 工艺技术 • 上一篇    下一篇

响应面法优化拟微绿球藻藻油脱色工艺

袁芝,朱孝晨,孙利芹   

  1. (烟台大学生命科学学院,山东?烟台 264005)
  • 出版日期:2017-12-25 发布日期:2017-12-07
  • 基金资助:
    山东省现代农业产业技术体系建设专项(SDAIT-26-03); 山东省2016年度农业重大应用技术创新项目(鲁财农指[2016]36号)

Optimization of Decolorization of Nannochloropsis oceanica Oil by Response Surface Methodology

YUAN Zhi, ZHU Xiaochen, SUN Liqin   

  1. (College of Life Sciences, Yantai University, Yantai 264005, China)
  • Online:2017-12-25 Published:2017-12-07

摘要: 以海洋微藻拟微绿球藻中提取的富含二十碳五烯酸的粗藻油为原料,探究脱色材料及脱色工艺对粗藻油脱色效果的影响。在单因素试验结果的基础上,以脱色剂用量、脱色温度、脱色时间为影响因素,以藻油脱色率和藻油回收率为响应值,应用Box-Behnken试验设计建立数学模型,进行响应面分析,优化脱色工艺条件。结果表明,活性炭对拟微绿球藻藻油的脱色效果最好,响应面优化的最佳脱色工艺条件为:活性炭用量3.8?g/100?mL、脱色温度68?℃、脱色时间54?min。在此条件下,微藻藻油的脱色率达到98.05%,经3?次解吸后,藻油总回收率达到81.45%。

关键词: 拟微绿球藻, 藻油, 脱色工艺, 响应面试验, 优化

Abstract: The optimization of the decolorization of crude oil extracted from Nannochloropsis oceanica, rich in eicosapentaenoic acid (EPA), was carried out using one-factor-at-a-time method and response surface methodology. Using a Box-Behnken design, a mathematical model was established indicating the effects of decolorizing agent dose, temperature and time on decolorization efficiency and oil recovery, respectively. The results showed that activated carbon exhibited the best decolorization efficiency. A decolorizing agent dose of 3.8 g/100 mL, a temperature of 68 ℃ and a decolorization time of 54 min were found to be the optimal conditions to obtain a higher decolorization efficiency of 98.05% and a higher oil recovery of 81.45% after three desorption cycles.

Key words: Nannochloropsis oceanica, microalgae oil, decolorization process, response surface methodology, optimization

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