食品科学

• 基础研究 • 上一篇    下一篇

玉米肽-锌螯合物结构表征及抗氧化活性分析

张 智1,刘 慧1,刘 奇1,郑喜群2,*,曲 巍1,杨可心1   

  1. 1.东北林业大学林学院,黑龙江 哈尔滨 150040;2.齐齐哈尔大学 黑龙江省普通高校农产品加工重点实验室,黑龙江 齐齐哈尔 161006
  • 出版日期:2017-02-15 发布日期:2017-02-28

Structural Characteristics and Antioxidant Activity of Corn Peptide-Zn Complex

ZHANG Zhi 1, LIU Hui1, LIU Qi1, ZHENG Xiqun2,*, QU Wei1, YANG Kexin1   

  1. 1. College of Forestry, Northeast Forestry University, Harbin 150040, China; 2. Key Constructive Laboratory of Processing Agricultural Products of Heilongjiang Province Normal University, Qiqihar University, Qiqihar 161006, China
  • Online:2017-02-15 Published:2017-02-28

摘要: 以玉米肽(corn peptide,CP)为参照研究玉米肽-锌(CP-Zn)螯合物的结构表征和体内抗氧化活性变化。采用氨基酸分析、紫外扫描、红外光谱研究结构变化;对小鼠灌胃不同剂量的CP、CP-Zn(低、中、高剂量组分别为50、150、250 mg/(kg·d)),测其血清、肝脏中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,分析比较CP、CP-Zn体内抗氧化活性。结果表明:螯合前后氨基酸组成、紫外吸收光谱、红外吸收光谱存在明显变化,Zn2+与—COOH、—NH2、C=O进行了配位;CP高剂量、CP-Zn高、中剂量能显著降低小鼠血清、肝脏中MDA含量(P<0.05或P<0.01),提高SOD活力(P<0.01)、GSH-Px活力(P<0.05或P<0.01),CP-Zn抗氧化活性优于CP。由此可见,CP-Zn属于螯合物,同时具有增强CP抗氧化活性的作用。

关键词: 玉米肽-锌螯合物, 结构, 抗氧化

Abstract: In this work, in comparison to corn peptide (CP), we investigated the change in structural characteristics and antioxidant activity in vivo of corn peptide-Zn (CP-Zn) complex. The structures of CP and CP-Zn were investigated through amino acid analysis, ultraviolet (UV) spectroscopy and infrared spectroscopy. CP and CP-Zn (50, 150, 250 mg/(kg·d)) at different doses were administered intragastrically to mice. Two days later, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity in serum and liver were measured to evaluate antioxidant activity in vivo of CP and CP-Zn. The results showed a significant change in amino acid content, and UV and FT-IR spectra between CP and CP-Zn. Zn2+ was coordinated with the –COOH, –NH2 and C=O groups of corn peptide. Compared with the control group, high-dose CP and high- and middle-dose CP-Zn could reduce MDA level (P < 0.05 or P < 0.01), and improve SOD (P < 0.01) and GSH-Px activity (P < 0.05 or P < 0.01) in serum and liver. The antioxidant activity of CP-Zn was better than that of CP.

Key words: corn peptide-Zn complex, structure, antioxidant activity

中图分类号: