食品科学 ›› 2017, Vol. 38 ›› Issue (6): 117-122.doi: 10.7506/spkx1002-6630-201706018

• 生物工程 • 上一篇    下一篇

响应面试验优化微波辅助固定化胰蛋白酶工艺

刘伟华,李 荣,姜子涛   

  1. 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    天津市自然科学基金重点项目(12JCZDJC34100);天津市高等学校创新团队培养计划项目(TD12-5049)

Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology

LIU Weihua, LI Rong, JIANG Zitao   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 在微波辅助的条件下,以环氧树脂为载体固定化胰蛋白酶。通过单因素试验和响应面分析法确定了微波辅助固定化胰蛋白酶的最佳条件:微波温度30 ℃、给酶量41 mg/g、pH 6.2、微波时间10 min。在此条件下制备的固定化胰蛋白酶活力为322.56 U/g树脂,高于传统振荡方法制备的固定化酶活力,将原来20 h的固定化时间缩短到几分钟,并且固定化酶具有良好的稳定性,由此可见微波辅助固定化酶是一种高效的固定化方法。

关键词: 固定化酶, 环氧树脂, 微波, 响应面, 高效

Abstract: Trypsin was immobilized onto epoxy resin under microwave irradiation. Using one-factor-at-a-time method and response surface methodology (RSM), the optimal conditions of immobilization were obtained as follows: trypsin dosage, 41 mg/g; microwave irradiation time, 10 min; pH, 6.2; and temperature, 30 ℃. Under these conditions, the activity of immobilized trypsin was 322.56 U/g resin, which was higher than that of traditional immobilized trypsin and the immobilization time was shortened from 20 h to a few minutes. Furthermore, immobilized trypsin had a good stability. Thus, microwave-assisted immobilization provides an efficient way of immobilizing trypsin.

Key words: immobilized trypsin, epoxy resin, microwave, response surface methodology, high efficiency

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