食品科学 ›› 2017, Vol. 38 ›› Issue (6): 153-159.doi: 10.7506/spkx1002-6630-201706024

• 生物工程 • 上一篇    下一篇

包埋培养法筛选白酒窖泥中一株功能细菌

宋连宝,赵 辉   

  1. 黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江 哈尔滨 150080
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    黑龙江省博士后科研启动基金项目(LBH-Q13139)

Screening of a Functional Bacterial Strain from Chinese Liquor Pit Mud by Microencapsulation Culture Method

SONG Lianbao, ZHAO Hui     

  1. Agricultural Microbiology Engineering Research Center, Ministry of Education, College of Life Science, Heilongjiang University, Harbin 150080, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 采用微包埋培养法结合流式细胞分选技术,对白酒窖泥中的细菌进行分离和筛选,分离到95 株细菌。经菌株生长实验从中筛选出8 株生长较缓慢的菌株LS4、LS27、LS32、LS37、LS64、LS66、LS69’、LS85,经气相色谱分析,LS66菌株产白酒香味物质较为丰富,确定其为出发菌株。对其进行形态学观察、生理生化和16S rDNA鉴定,该菌株可能为一株窖泥未培养功能菌株uncultured bacterium clone N8-7,对该菌株的培养条件进行初步研究,菌株生长的最适温度、最适pH值和最佳发酵接种量分别为35 ℃、pH 6.5和4%。

关键词: 白酒, 窖泥, 细菌, 微包埋, 未培养

Abstract: A total of 95 bacterial strains were isolated from Chinese liquor pit mud by the microencapsulation culture method through cell sorting using flow cytometry. Out of these isolates, the strains LS4, LS27, LS32, LS37, LS64, LS66, LS69’ and LS85 were selected for slow growth rates. Gas chromatography analysis demonstrated that LS66 was determined as the best one for the production of an abundance of aroma compounds in Chinese liquor. By morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis, it was tentatively identified as an uncultured functional bacterium, termed N8-7. The optimal culture temperature, pH and inoculums size for this strain were determined to be 35 ℃, 6.5 and 4%, respectively.

Key words: liquor, pit mud, bacteria, microencapsulation, uncultured

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