食品科学 ›› 2017, Vol. 38 ›› Issue (6): 236-241.doi: 10.7506/spkx1002-6630-201706037

• 工艺技术 • 上一篇    下一篇

西藏野生卷叶黄精多酚的提取及其抗氧化活性分析

张国强,郭晓东,薛文华,兰小中   

  1. 西藏农牧学院,西藏 林芝 860000
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    国家自然科学基金地区科学基金项目(31260371);西藏特色农牧资源协同创新中心-藏药材项目(2014-2016);西藏自治区科技厅重点科研项目(2016-2017);西藏农牧学院青年骨干教师支持计划项目(2015XYD08)

Extraction and Antioxidant Activities of Polyphenols from Tibetan Wild Polygonatum cirrhifolium

ZHANG Guoqiang, GUO Xiaodong, XUE Wenhua, LAN Xiaozhong   

  1. XiZang Agriculture and Animal Husbandry College, Linzhi 860000, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 以西藏野生卷叶黄精为原料,用乙醇提取其中的多酚类物质,通过单因素试验和正交试验,探讨提取温度、料液比、乙醇溶液体积分数和提取时间对多酚提取效果的影响并确定最佳提取工艺。利用DPPH自由基、超氧阴离子自由基、羟自由基活性检测法,研究其抗氧化活性。结果表明:提取温度70 ℃、料液比1∶25(g/mL)、乙醇溶液体积分数70%、提取时间2.5 h时,多酚提取量最大,达到19.8 mg/g;以VC为对照,在最优工艺条件下测定多酚提取物清除能力,多酚提取物具有较强的抗氧化活性,是一种极具潜力的天然抗氧化剂。

关键词: 卷叶黄精, 多酚, 提取, 抗氧化活性

Abstract: This study aimed to optimize the extraction of polyphenols from Titeban wild Polygonatum cirrhifolium using ethanol as the extraction solvent. Towards this goal, one-factor-at-a-time method and orthogonal array design were employed to analyze the effects of extraction temperature, solid to liquid ratio, ethanol concentration, and extraction time on extraction efficiency. Furthermore, the antioxidant activities of polyphenol extracts were assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl free radical scavenging assays. The results showed the maximum extraction efficiency of polyphenols was achieved after 2.5 h extraction using 70% ethanol as the extraction solvent at a solid to solvent ratio of 1:25 (g/mL) at 70 ℃. The polyphenol extracts from P. cirrhifolium obtained under the optimized conditions had strong free radical scavenging capacities in comparison with VC, thus being a potential source of natural antioxidants.

Key words: Polygonatum cirrhifolium, polyphenols, extraction, antioxidant activity

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