改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14 种非法添加工业染料

doi:10.7506/spkx1002-6630-201708046

食品科学 ›› 2017, Vol. 38 ›› Issue (8): 296-302.doi: 10.7506/spkx1002-6630-201708046

改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14 种非法添加工业染料

陈林，温家欣，吴霞，雷毅，张荣

1.南阳市食品药品检验所，河南南阳 473059；2.广东省药品检验所，广东广州 510180； 3.安捷伦科技有限公司，广东广州 510613；4.广东药科大学药科学院，广东广州 510006

出版日期:2017-04-25 发布日期:2017-04-24
基金资助:
广东省省级科技计划项目（2013B090200059）；广州市健康产品非法添加化学成分快速检测技术重点实验室项目（2013年）；广东省食品药品检验检测技术创新专项（2015ZX01）

Rapid Simultaneous Determination of 14 Adulterated Industrial Dyes in Chilli Products by a Modified QuEChERS Method Coupled with LC-MS/MS

CHEN Lin, WEN Jiaxin, WU Xia, LEI Yi, ZHANG Rong

1. Nanyang Institute for Food and Drug Control, Nanyang 473059, China; 2. Guangdong Institute for Drug Control, Guangzhou 510180, China; 3. Agilent Technologies Co. Ltd., Guangzhou 510613, China; 4. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China

Online:2017-04-25 Published:2017-04-24

摘要/Abstract

摘要： 采用改良QuEChERS技术作为前处理方法，建立了辣椒制品中14 种非法添加工业染料的液相色谱-串联质谱联用快速检测方法。样品采用乙腈-丙酮（7∶3，V/V）提取，经EMR-Lipid净化，以Agilent Poroshell 120 EC C18（50 mm×2.1 mm，2.7 μm）色谱柱分离，采用多反应监测模式检测，外标法定量分析。14 种成分在11 min内完成分离，检出限为0.4～7.1 μg/kg，不同加标水平的平均回收率为70.5%～102.1%，相对标准偏差为0.3%～9.3%。本方法准确、快速、重复性好，可为辣椒制品中非法添加工业染料的检测提供一种更加快速、简便的技术支持。

关键词: QuEChERS, EMR-Lipid, 液相色谱-串联质谱联用, 辣椒制品, 非法添加工业染料

Abstract: A rapid method for the simultaneous determination of 14 adulterated industrial dyes in chilli products was developed by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) based on a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample pretreatment method. Samples were extracted with acetonitrile-acetone (7:3, V/V), and then the extract was cleaned up using Enhanced Matrix Removal Lipid (EMR-Lipid). The chromatographic separation was performed on an Agilent Poroshell 120 EC C18 (50 mm × 2.1 mm, 2.7 μm) column. The separated analytes were analyzed with LC-MS/MS by multiple reaction monitoring (MRM), and quantified by the external standard method. The results showed that the 14 pigments were separated completely in 11 minutes, and their quantitative detection limits ranged from 0.4 to 7.1 μg/kg. The average recoveries at three spiked levels ranged from 70.5% to 102.1% with relative standard deviations (RSDs)(n = 6) of 0.3%–9.3%. The method is quick, accurate, and sensitive, and can be applied to the determination of 14 adulterated industrial dyes in chilli products.

Key words: QuEChERS, EMR-lipid, LC-MS/MS, chilli products, adulterated industrial dyes

中图分类号:

O658

陈林，温家欣，吴霞，雷毅，张荣. 改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14 种非法添加工业染料[J]. 食品科学, 2017, 38(8): 296-302.

CHEN Lin, WEN Jiaxin, WU Xia, LEI Yi, ZHANG Rong. Rapid Simultaneous Determination of 14 Adulterated Industrial Dyes in Chilli Products by a Modified QuEChERS Method Coupled with LC-MS/MS[J]. FOOD SCIENCE, 2017, 38(8): 296-302.

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改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14 种非法添加工业染料

Rapid Simultaneous Determination of 14 Adulterated Industrial Dyes in Chilli Products by a Modified QuEChERS Method Coupled with LC-MS/MS

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