食品科学 ›› 2017, Vol. 38 ›› Issue (8): 49-55.doi: 10.7506/spkx1002-6630-201708009

• 生物工程 • 上一篇    下一篇

不同茶类发花茯茶中“金花”菌的分离、鉴定及产黄曲霉毒素分析

黄 浩,,郑红发,赵 熙,银 霞,黄怀生,粟本文,钟兴刚,黄建安,刘仲华,   

  1. 1.湖南省农业科学院茶叶研究所,湖南 长沙 410125;2.湖南农业大学 茶学教育部重点实验室,湖南 长沙 410128; 3.国家植物功能成分利用工程技术研究中心,湖南 长沙 410128
  • 出版日期:2017-04-25 发布日期:2017-04-24
  • 基金资助:
    湖南省自然科学基金(湖南省科学技术厅与湖南省农业科学院联合基金)项目(2016JJ6058); 国家自然科学基金面上项目(31471706);国家现代农业(茶叶)产业技术体系建设专项(CARS-23); 湖南省茶叶研究所所长基金项目(2015SJ01)

Identification and Aflatoxin Production of ‘Golden Flora’ Fungi Isolated from Fu Tea Produced from Different Kinds of Tea

HUANG Hao,, ZHENG Hongfa, ZHAO Xi, YIN Xia, HUANG Huaisheng, SU Benwen,ZHONG Xinggang, HUANG Jian’an, LIU Zhonghua,   

  1. 1. Tea Research Institute, Hunan Academy Agricultural Sciences, Changsha 410125, China; 2. Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China; 3. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
  • Online:2017-04-25 Published:2017-04-24

摘要: 以乌龙茶(大红袍)、红茶、绿茶、黑茶(天尖原料、金湘益茯砖茶原料)5 种茶样为原料,运用“散茶发花”技术制得不同茶类散装茯茶,基于各成品茶中“金花”菌间存在一定的形态差异,运用微生物手段对各茯茶制品中“金花”菌进行差异分析,同时从形态与DNA序列分析角度对其进行了系统的鉴定,并运用高效液相色谱-三重四极杆串联质谱对其是否具备产黄曲霉毒素的能力进行检测分析。结果表明:不同茶类发花茯茶成品中的“金花”菌实属同一种真菌,无异于原接种供试“金花”菌,该菌被鉴定为真菌门(Eumycota;Mycobionta)、子囊菌纲(Ascomycetes)、真子囊菌亚纲(Euascomycetes)、曲霉目(Eurotiales)、曲霉科(Eurotiaceae)、散子囊菌属(Eurotium)、冠突散囊菌(Eurotium cristatum),无性型为针刺曲霉(Aspergillus spiculosus Blaser);此外,质谱数据显示未检测出黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1和黄曲霉毒素G2,表明该分离“金花”菌不具备产黄曲霉毒素的能力。

关键词: 茯茶, 散茶发花, “金花”菌, 鉴定, 冠突散囊菌, 黄曲霉毒素

Abstract: Five different kinds of Fu tea were produced from oolong tea (Dahongpao), black tea, green tea, and tea leaves used for making two kinds of dark tea, Tianjian tea and Jinxiangyi brick tea, by fungal fermentation in a loose state. ‘Golden Floral’ fungi isolated from these Fu teas showed morphological differences. Further differences were investigated by microbiological methods. The isolated fungi were systematically identified by morphology and DNA sequence analysis. Meanwhile, the aflatoxin producing ability of these isolates was determined by high performance liquid chromatography (HPLC)-triple quadrupole tandem mass spectrometry. The results revealed that all the ‘Golden Floral’ fungi isolated were assigned to the same species and they were not different from the initial inoculum, which were classified as Eurotium cristatum in the Eumycota genus in the Eurotiaceae family in the Eurotiales order in the Euascomycetes subclass in the Ascomycetes class in the Eumycota phylum and its anamorph was identified as Aspergillus spiculosus Blaser. And the mass spectral data showed that AFB1, AFB2, AFG1 and AFG2 were not detected, indicating that the ‘Golden Floral’ fungi are not able to produce aflatoxins.

Key words: Fu tea, fungal fermentation of tea in a loose state, ‘Golden Floral’ fungi, identification, Eurotium cristatum, aflatoxin

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