食品科学 ›› 2017, Vol. 38 ›› Issue (9): 133-137.doi: 10.7506/spkx1002-6630-201709021

• 基础研究 • 上一篇    下一篇

大气滑动弧放电对沙门氏菌的灭活机制及在鸡蛋保鲜中的应用

朱莉华,李 燕,仝其根,孙运金,马挺军   

  1. 1.北京农学院食品科学与工程学院,食品质量与安全北京实验室,北京 102206;2.北京农学院食品科学与工程学院,农产品有害微生物及农残安全检测与控制北京市重点实验室,北京 102206
  • 出版日期:2017-05-15 发布日期:2017-05-22

Inactivation Mechanism of Salmonella by Atmospheric Gliding Arc Discharge and Its Application in the Preservation of Eggs

ZHU Lihua, LI Yan, TONG Qigen, SUN Yunjin, MA Tingjun   

  1. 1. Beijing Laboratory of Food Quality and Safety, College of Food Science and Engineering, Beijing University of Agriculture,Beijing 102206, China; 2. Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms andPesticides, College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China
  • Online:2017-05-15 Published:2017-05-22

摘要: 为了保持鸡蛋的新鲜度、延长其保质期,本研究提出了一种可在室温和大气环境中进行灭菌处理的新型冷杀菌技术,即滑动弧放电技术。首先,将配制好的沙门氏菌液均匀接种在鸡蛋表面,然后使鸡蛋以一定的自旋速率通过等离子体放电区域进行灭菌处理,从鸡蛋表面取得菌样后进行灭活机制研究,并将灭菌处理后的鸡蛋在37 ℃条件下进行贮藏,通过测定质量损失率和哈夫单位来评价大气放电技术对鸡蛋品质的影响。扫描电子显微镜观察和细胞外蛋白质质量浓度检测结果表明,大气滑动弧放电技术可对沙门氏菌产生有效的刻蚀作用,导致细胞膜破碎和胞内蛋白质泄漏。同时,鸡蛋品质检测表明,滑动弧放电处理组无论作用时间长短,鸡蛋质量损失率增大的程度均比对照组明显,但哈夫单位的降低程度小于对照组,说明大气滑动弧放电可在一定程度保持鸡蛋的新鲜度。

关键词: 大气滑动弧放电, 鸡蛋, 保鲜, 灭活, 沙门氏菌

Abstract: In order to retain the freshness and prolong the shelf-life of fresh eggs, a new type of cold sterilization technology was put forward, namely gliding arc discharge, which can be worked in air environment at room temperature. Firstly, egg surface was coated with Salmonella suspension and transported through plasma discharge plume at a certain speed of self-spinning to inactivate the surface bacteria, part of which was sampled to investigate its inactivated mechanism and the remaining treated eggs were stored in a sealed environment at 37 ℃ to assess the impact of this air discharge plasma on egg quality by measuring the mass loss rate and Haugh unit (HU). The results of scanning electron microscopy (SEM) and extracellular protein concentration analysis indicate that atmospheric gliding arc discharge technique could have an effective etching effect on Salmonella, leading to cell membrane disruption and leakage of intracellular proteins of Salmonella. Meanwhile, the egg quality measurements showed that the atmospheric gliding arc discharge could maintain the freshness of eggs to a certain extent.

Key words: atmospheric gliding arc discharge, fresh eggs, preservation, inactivation, Salmonella

中图分类号: