短梗五加果多酚预防大鼠动脉粥样硬化作用

doi:10.7506/spkx1002-6630-201801030

食品科学 ›› 2018, Vol. 39 ›› Issue (1): 200-206.doi: 10.7506/spkx1002-6630-201801030

短梗五加果多酚预防大鼠动脉粥样硬化作用

何忠梅1，李成恩1，段翠翠2，赵玉娟2，高磊2，栾畅2，张连学1，李盛钰2,*

1.吉林农业大学中药材学院，吉林长春 130118；2.吉林省农业科学院农产品加工研究所，吉林长春 130033

出版日期:2018-01-15 发布日期:2018-01-05
基金资助:
长春市产学研协同创新示范点建设项目（16CX20）；国家现代农业（奶牛）产业技术体系建设专项（CARS-36）； “十三五”国家重点研发计划重点专项（2016YFC0500300）；吉林省中医药产业发展专项（20140311029YY）

Preventive Effect of Polyphenols Isolated from Acanthopanax sessiliflorus Fruits on Atherosclerosis in Rats

HE Zhongmei1, LI Cheng’en1, DUAN Cuicui2, ZHAO Yujuan2, GAO Lei2, LUAN Chang2, ZHANG Lianxue1, LI Shengyu2,*

1. College of Traditional Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China;2. Institute of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China

Online:2018-01-15 Published:2018-01-05

摘要/Abstract

摘要： 目的：研究短梗五加果多酚（Acanthopanax sessiliflorus fruit polyphenols，ASFP）对大鼠动脉粥样硬化（atherosclerosis，As）的预防作用，并探讨其作用机制。方法：40 只雄性SD大鼠随机平均分成4 组，每组10 只。采用高脂饮食结合腹腔注射VD3的方法建立As模型，不同剂量ASFP给药组自造模开始之日起分别灌胃150、 75 mg/（kg·d）的ASFP，空白组和高脂饮食组大鼠灌胃等量生理盐水，各实验组连续处理12 周后，检测大鼠血清生理生化水平并计算As指数；苏木素-伊红染色观察大鼠主动脉As病变情况；Western blot检测主动脉中黏附分子和丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）信号通路关键基因的蛋白表达水平。结果：与高脂饮食组相比，ASFP能显著降低大鼠血清中的血脂、黏附分子和炎症因子水平，减少主动脉中的脂质沉积，改善各层结构排列紊乱，显著降低主动脉中细胞间黏附分子-1、血管细胞黏附分子-1、磷酸化p38和磷酸化细胞外信号调节蛋白激酶1/2（phospho-extracellular signal regulated kinases 1/2，p-ERK1/2）的蛋白表达量。结论：ASFP具有预防 As的作用，其作用可能与其降血脂和抑制p38 MAPK和ERK1/2 MAPK信号通路，进而抑制炎症因子的表达有关。

关键词: 短梗五加果, 多酚, 动脉粥样硬化, 降血脂, 抗炎

Abstract: Objective: A rat model was used to investigate the preventive effect of Acanthopanax sessiliflorus fruit polyphenols (ASFP) on atherosclerosis (As) and the underlying mechanism was explored. Methods: The atherosclerosis model was built by feeding rats with high-fat diet (HFD) combined with intraperitoneal injection with vitamin D3. Forty male rats were randomly divided into four groups (n = 10): control, HFD, HFD + low-dose ASFP and HFD + high-dose ASFD groups. The rats from the ASFD-treat groups were daily administered with 150 or 75 mg/(kg·d) ASFP during the HFD feeding period of 12 days, while those from the control and HFD groups were given an equal volume of normal saline. Serum biochemical parameters were detected and atherosclerosis index (AI) was calculated at the end of administration. Hematoxylin-eosin (HE) staining was used to observe the atherosclerosis lesion in the aorta of rats. The expression levels of adhesion molecules in the aorta and key genes involved mitogen-activated protein kinase (MAPK) signaling pathway were measured by Western blot. Results: The levels of blood lipids, adhesion molecules and inflammatory cytokines in rats were significantly decreased by ASFP compared with the model group. In addition, after treatment with ASFP, aortic lipid deposition was reduced and the structure of disordered layers was significantly improved compared with the model group. Furthermore, the expression of intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), phospho-p38 (p-p38) and phospho-ERK1/2 (p-ERK1/2) in the rat aorta were significantly down-regulated by treatment with ASFP compared with the model group. Conclusion: ASFP plays an important role in preventing atherosclerosis, which may be related to the reduction of blood lipids and the inhibition of p38 MAPK and ERK1/2 MAPK signaling pathways, thereby inhibiting the expression of inflammatory cytokines.

Key words: Acanthopanax sessiliflorus, polyphenols, atherosclerosis, blood lipid-lowering, anti-inflammatory

中图分类号:

TS201.4

何忠梅，李成恩，段翠翠，赵玉娟，高磊，栾畅，张连学，李盛钰. 短梗五加果多酚预防大鼠动脉粥样硬化作用[J]. 食品科学, 2018, 39(1): 200-206.

HE Zhongmei, LI Cheng’en, DUAN Cuicui, ZHAO Yujuan, GAO Lei, LUAN Chang, ZHANG Lianxue, LI Shengyu. Preventive Effect of Polyphenols Isolated from Acanthopanax sessiliflorus Fruits on Atherosclerosis in Rats[J]. FOOD SCIENCE, 2018, 39(1): 200-206.

[1]	李彩云，李洁，严守雷，王清章. 果蔬酶促褐变机理的研究进展[J]. 食品科学, 2021, 42(9): 283-292.
[2]	詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.
[3]	李文峰，张向阳，王翠，林兰婷，陈小平，屈阳，张雪梅，林瑶，谭飔，郑俏然，高晓旭. 茎瘤芥的气体射流冲击干燥动力学及多酚降解动力学特征[J]. 食品科学, 2021, 42(5): 106-114.
[4]	陈慧, YOSHINORI Katakura, 扈洪波, 尹淑涛, 赵冲. SIRT3多酚激活剂的筛选及其对UVB诱导HaCaT细胞衰老的修复作用[J]. 食品科学, 2021, 42(5): 115-121.
[5]	李伟，叶嘉宜，陈运娇，曹庸. 桉叶多酚提取物体内外抗氧化活性评价[J]. 食品科学, 2021, 42(5): 160-168.
[6]	种俸亭，黄子珍，滕建文，韦保耀，黄丽，夏宁. 百香果皮体外抑制葡萄糖吸收、抗氧化及调节高血糖大鼠肠道菌群结构的作用[J]. 食品科学, 2021, 42(5): 193-200.
[7]	范三红，王娇娇，李佳妮，白宝清. 辣椒红色素和辣椒碱体内抗氧化活性及降血脂功能[J]. 食品科学, 2021, 42(5): 201-207.
[8]	赵媚，常凌，宋泽和，贺喜. 植物多酚与肠道微生物群的相互作用及其对代谢性疾病影响的研究进展[J]. 食品科学, 2021, 42(5): 305-313.
[9]	康子悦，沈蒙，葛云飞，王娟，全志刚，肖金玲，王维浩，曹龙奎. 基于植物广泛靶向代谢组学技术探究小米粥中酚类化合物组成及其抗氧化性[J]. 食品科学, 2021, 42(4): 206-214.
[10]	徐艳阳，赵玉娟，高峰，王二雷，鲁海玲，李雪凤，姜雯雯，陈艳. 高效液相色谱法分析中国人参不同部位中多酚类化合物[J]. 食品科学, 2021, 42(4): 240-246.
[11]	高浩祥，陈南，徐乾达，何强，曾维才. 油炸过程中茶多酚对油脂品质的影响[J]. 食品科学, 2021, 42(4): 1-7.
[12]	周雅琪，黄佳茵，陈美玉，葛雨珺，李苑，胡亚芹. 亚牛磺酸对南美白对虾多酚氧化酶活性及酶构象的影响[J]. 食品科学, 2021, 42(4): 100-106.
[13]	魏征，郭咪咪，王雅朦，段章群，杨剀舟，栾霞. 油茶籽油多酚化合物研究进展[J]. 食品科学, 2021, 42(3): 311-320.
[14]	冉丽丹，李文慧，赵超，钟媛媛，袁湖川，闫青青，朱伟超，董娟. 茶多酚-β-环糊精包合物对羊肚冷藏期间肌原纤维蛋白氧化的影响[J]. 食品科学, 2021, 42(3): 227-235.
[15]	马娜，王星晨，孔彩琳，陶永胜. 胶红酵母与酿酒酵母共发酵对干红葡萄酒香气与色泽的影响[J]. 食品科学, 2021, 42(2): 97-104.

短梗五加果多酚预防大鼠动脉粥样硬化作用

Preventive Effect of Polyphenols Isolated from Acanthopanax sessiliflorus Fruits on Atherosclerosis in Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价