食品科学 ›› 2018, Vol. 39 ›› Issue (22): 87-92.doi: 10.7506/spkx1002-6630-201822014

• 生物工程 • 上一篇    下一篇

枯草芽孢杆菌ZJS18发酵生产γ-聚谷氨酸培养条件的优化

于平,黄星星,张一舒   

  1. (浙江工商大学食品与生物工程学院,浙江?杭州 310035)
  • 出版日期:2018-11-25 发布日期:2018-11-21
  • 基金资助:
    国家自然科学基金面上项目(31171658);食品科学与工程浙江省重中之重一级学科项目(2017SIAR214)

Optimization of Culture Conditions for Poly γ-Glutamic Acid Production by Bacillus subtilis ZJS18

YU Ping, HUANG Xingxing, ZHANG Yishu   

  1. (College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China)
  • Online:2018-11-25 Published:2018-11-21

摘要: 通过响应面法对枯草芽孢杆菌ZJS18发酵生产γ-聚谷氨酸的培养条件进行优化。首先采用Plackett-Burman试验设计筛选出对γ-聚谷氨酸产量有显著影响的3?个关键因素,即蔗糖、酵母粉和谷氨酸钠;然后通过Box-Behnken试验设计和响应面法对这3?个关键因素的用量进行优化。响应面优化后的3?个关键因素的最佳质量浓度为蔗糖64.40?g/L、酵母粉7.10?g/L和谷氨酸钠57.96?g/L。枯草芽孢杆菌ZJS18发酵生产γ-聚谷氨酸的最佳培养条件为蔗糖用量64.40?g/L、酵母粉用量7.10?g/L、谷氨酸钠用量57.96?g/L,氯化钠用量30?g/L,MgSO4用量0.3?g/L、K2HPO4用量2?g/L,初始pH?7.5,接种量5%,装液量40?mL/250?mL,温度37?℃,摇床转速200?r/min,发酵时间36?h。在上述条件下,γ-聚谷氨酸产量为13.20?g/L。与未优化前相比,产量提高了1.88?倍。

关键词: 枯草芽孢杆菌, γ-聚谷氨酸, 培养条件, 响应面法, 优化

Abstract: The culture conditions for poly γ-glutamic acid production by Bacillus subtilis ZJS18 were optimized by response surface methodology (RSM). Three factors that had a significant influence on the yield of poly γ-glutamic acid, namely sucrose, yeast powder and sodium glutamate, were screened out by the Plackett-Burman design. The levels of the three medium components were further optimized to be 64.40, 7.10 and 57.96 g/L, respectively using Box-Behnken design with RSM. The optimal culture conditions for the production of poly γ-glutamic acid were as follows: sucrose 64.40 g/L, yeast powder 7.10 g/L, sodium glutamate 57.96 g/L, NaCl 30 g/L, KH2PO4 2 g/L, MgSO4 0.3 g/L, initial pH 7.5, inoculum size 5%, medium volume 40 mL/250 mL, temperature 37 ℃, shaking speed 200 r/min, and fermentation time 36 h. Under the above conditions, the yield of poly γ-glutamic acid was up to 13.20 g/L, which was increased by 2.88 folds as compared to that under the original conditions.

Key words: Bacillus subtilis, poly γ-glutamic acid, culture conditions, response surface methodology, optimization

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