食品科学 ›› 2018, Vol. 39 ›› Issue (4): 125-131.doi: 10.7506/spkx1002-6630-201804019

• 生物工程 • 上一篇    下一篇

响应面法优化木聚糖酶和α-葡萄糖醛酸酶联合水解桦木木聚糖工艺

董媛媛,李亚贤,沈艺红,薛业敏*   

  1. (南京师范大学金陵女子学院,江苏南京 210097)
  • 出版日期:2018-02-25 发布日期:2018-02-02
  • 基金资助:
    淮安市重点研发计划项目(HAN201621)

Optimization of Hydrolysis Conditions of Xylanase and α-Glucuronidase Mixture for Birch Xylan by Response Surface Methodology

DONG Yuanyuan, LI Yaxian, SHEN Yihong, XUE Yemin*   

  1. (Ginling College, Nanjing Normal University, Nanjing 210097, China)
  • Online:2018-02-25 Published:2018-02-02

摘要: 在单因素试验基础上应用响应面试验,优化重组耐热性木聚糖酶(XynB)和α-葡萄糖醛酸酶(AguA)联合水解桦木木聚糖的条件。响应面法分析结果显示,4个影响因素的最佳组合为底物质量浓度4.2g/100mL、酶解温度80.66℃、pH7.65、XynB/AguA加酶量60/9U/g,此时还原糖释放量为17.82mg/mL。利用木聚糖酶和葡萄糖醛酸酶共同作用木聚糖4h所得低聚木糖中还原糖质量浓度为17.91mg/mL,木二糖质量浓度为13.66mg/mL。

关键词: 木聚糖酶, α-葡萄糖醛酸酶, 木聚糖水解, 响应面试验, 优化

Abstract: Objective: This study aimed to optimize the hydrolysis of birch xylan with a mixture of recombinant thermostable xylanase (XynB) and glucuronidase (AguA) from an engineered strain of Escherichia coli. Methods: The hydrolysis conditions were optimized using combination of one-factor-at-a-time method and response surface methodology. Results: The optimized conditions were obtained as follows: substrate concentration, 4.2 g/100 mL; temperature, 80.66 ℃; pH, 7.65; and XynB/AguA dosage, 60/9 U/g, yielding a reducing sugar concentration of 17.82 mg/mL. Conclusion: The reducing sugar and xylose concentrations were respectively 17.91 mg/mL and 13.66 mg/mL after 4 h of hydrolysis with the enzyme mixture.

Key words: xylanase (XynB), α-glucuronidase (AguA), hydrolysis of xylan, response surface methodology, optimization

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