食品科学 ›› 2018, Vol. 39 ›› Issue (4): 194-198.doi: 10.7506/spkx1002-6630-201804029

• 成分分析 • 上一篇    下一篇

植物源食品中α-胡萝卜素和β-胡萝卜素的测定及比较分析

崔亚娟1,2,孔凡华2,刘国杰1,*,鲁绯2,李东2   

  1. (1.中国农业大学园艺学院,北京 100094;2.北京市营养源研究所,北京市系统营养工程技术研究中心,北京 100069)
  • 出版日期:2018-02-25 发布日期:2018-02-02
  • 基金资助:
    首都食品安全科技创优培育专项(Z141100002614013)

Determination and Comparison of α-Carotene and β-Carotene of Plant-Derived?Foods

CUI Yajuan1,2, KONG Fanhua2, LIU Guojie1,*, LU Fei2, LI Dong2   

  1. (1. College of Horticulture, China Agricultural University, Beijing 100094, China; 2. Beijing Institute of Nutrition Resources, Beijing System Nutrition Engineering and Technology Research Center, Beijing 100069, China)
  • Online:2018-02-25 Published:2018-02-02

摘要: 采用高效液相色谱法建立植物源食品中α-胡萝卜素和β-胡萝卜素同时在线分析检测技术,并以胡萝卜冻干粉为样品进行方法学验证。结果显示,α-胡萝卜素和β-胡萝卜素分别在0.8~20、0.4~8.2μg/mL范围内线性良好,精密度的相对标准偏差分别为4.45%、4.71%,加标回收率分别为96%~112%、83.5%~103%,加标回收率的相对标准偏差分别为2.67%~5.83%、4.90%~7.42%,以上结果说明该方法重复性良好、准确可行。采用该方法测定了多种植物源食品的胡萝卜素含量并进行比较分析,大多数样品中β-胡萝卜素含量高于α-胡萝卜素,只有南瓜中所含的α-胡萝卜素高于β-胡萝卜素。

关键词: 高效液相色谱法, 胡萝卜素, α-胡萝卜素, β-胡萝卜素, 植物源食品

Abstract: The study was conducted to develop an analytical method for simultaneous online determination of α-carotene and β-carotene in plant-derived?foods by high performance liquid chromatography (HPLC). Furthermore, the method was validated with freeze-dried carrot powder. The results showed that the calibration curves for α-carotene and β-carotene were linear in the ranges of 0.8–20 and 0.4–8.2 μg/mL, respectively. The precision expressed as relative standard deviation (RSD) were 4.45% and 4.71%, respectively. The recoveries at three spiked levels were 96%–112% and 83.5%–103% with RSD values of 2.67%–5.83% and 4.90%–7.42%, respectively. These data indicated good repeatability and accuracy. Comparative analysis of various plant-derived?foods by this method showed that β-carotene was more abundant than α-carotene in all samples except for pumpkin, which gave the opposite result.

Key words: high-performance liquid chromatography, carotene, α-carotene, β-carotene, plant-derived?foods

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