食品科学 ›› 2018, Vol. 39 ›› Issue (6): 207-214.doi: 10.7506/spkx1002-6630-201806033

• 成分分析 • 上一篇    下一篇

UPLC-PDA-MS/MS测定红瓤核桃中花青苷类物质

李永洲1,2,尚军华1,周奕菲1,吴文江3,揭波1,吴国良1,2,*   

  1. (1.河南农业大学园艺学院,河南?郑州 450002;2.河南省果树瓜类生物学重点实验室,河南?郑州 450002;3.河南农业大学食品科学技术学院,河南?郑州 450002)
  • 出版日期:2018-03-25 发布日期:2018-03-14
  • 基金资助:
    农业部果树育种技术重点实验室开放课题(NYB-2015-08-4);国家林业局核桃资源调查与编目项目(GR-2015-06)

Determination of Anthocyanins in Red-Fleshed Walnut by Ultra Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

LI Yongzhou1,2, SHANG Junhua1, ZHOU Yifei1, WU Wenjiang3, JIE Bo1, WU Guoliang1,2,*   

  1. (1. College of Horticultural Science, Henan Agricultural University, Zhengzhou 450002, China;2. Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou 450002, China;3. College of Food Sciences and Technology, Henan Agricultural University, Zhengzhou 450002, China)
  • Online:2018-03-25 Published:2018-03-14

摘要: 采用超高效液相色谱-二极管阵列检测器串联质谱定性和定量检测方法对不同时期的红瓤核桃树叶片及果皮提取物进行初步分析。样品选取于河南农业大学园艺学院果树试验站,从发芽初期开始定期采红瓤核桃的叶片,采样分为3?个时期(发芽初期、生长期、结果期),绿皮普通核桃叶片为对照,在果实成熟期分别取红瓤核桃和普通核桃果皮。液相色谱与质谱的检测结果表明:8?种目标化合物的定量限为0.60~0.90?μg/mL,在0~300?μg/L范围内呈现良好的线性关系,相关系数(R2)为0.999?0~0.999?6。采用该方法对不同时期的红瓤核桃叶片和果皮与普通核桃叶片和果皮进行分析,发现核桃果皮和叶片中的主要花青苷类物质的成分和含量的差异有统计学意义。该方法具有易操作、灵敏度高、重复性好、花青苷类物质的分析覆盖面广的特点,可用于核桃中花青苷类成分快速鉴定及含量测定。

关键词: 红瓤核桃叶片和果皮, 超高效液相色谱-二极管阵列检测器串联质谱, 花青苷

Abstract: This study was concerned with the identification and quantification of anthocyanins in tree leaves and peel of red-fleshed walnut harvested at different growing stages (early sprouting, leaf growth and fruiting) by ultra performance liquid chromatography with photodiode array detection coupled with electrospray ionization tandem mass spectrometry (UPLC-PDA-MS/MS). The samples were from the Fruit Experimental Station of the College of Horticulture of Henan Agricultural University. Leaves of ordinary walnut were used as control. The results showed that the limits of quantification (LOQs) for eight target compounds were in the range of 0.60–0.90 μg/mL. The calibration curves were linear in the range of 0–300 μg/L with correlation coefficient (R2) of 0.999 0–0.999 6. Walnut peel and leaves were statistically significantly different with respect to major anthocyanins, as determined by UPLC-PDA-MS/MS. The analytical method proved to be easy to operate, sensitive and repeatable and thus was useful for rapid identification and quantification of a variety of anthocyanins in walnut materials.

Key words: leaves and peel of red-fleshed walnut, UPLC-PDA-MS/MS, anthocyanins

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