食品科学 ›› 2019, Vol. 40 ›› Issue (17): 30-35.doi: 10.7506/spkx1002-6630-20180727-328

• 基础研究 • 上一篇    下一篇

两种饲养方式下苏尼特羊肉的氧化稳定性

罗玉龙,刘畅,李文博,王柏辉,窦露,杜瑞,要铎,赵丽华,苏琳,靳烨   

  1. (内蒙古农业大学食品科学与工程学院,内蒙古?呼和浩特 010018)
  • 出版日期:2019-09-15 发布日期:2019-09-23
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660439);“十三五”国家重点研发计划重点专项(2016YFE0106200); 内蒙古传统发酵食品加工与安全关键技术研究项目;内蒙古自然科学基金面上项目(2018MS03050)

Effects of Two Feeding Patterns on Oxidation Stability of Sunit Sheep Meat

LUO Yulong, LIU Chang, LI Wenbo, WANG Bohui, DOU Lu, DU Rui, YAO Duo, ZHAO Lihua, SU Lin, JIN Ye   

  1. (College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)
  • Online:2019-09-15 Published:2019-09-23

摘要: 以舍饲、放牧两种饲养方式下的12?月龄苏尼特羊股二头肌为实验材料,分别测定脂质氧化产物含量、硫代巴比妥酸值、抗氧化能力、抗氧化酶活力以及抗氧化酶相关调控基因表达量等指标并进行比较分析,旨在探索两种饲养方式下苏尼特羊肉的氧化稳定性。结果表明:放牧饲养苏尼特羊肉TBA值高度显著低于舍饲饲养(P<0.001);羊肉中主要的脂质氧化产物为己醛、庚醛、壬醛、1-辛烯-3-醇以及2,3-辛二酮,其含量均在放牧饲养羊肉中显著较低(P<0.05),表明舍饲饲养羊肉的脂质氧化程度相比放牧饲养羊肉更严重。放牧饲养羊肉的总抗氧化能力(P<0.01)、铜离子还原能力(P<0.05)和超氧化物歧化酶(superoxide dismutase,SOD)(P<0.001)、过氧化氢酶(catalase,CAT)(P<0.05)、谷胱甘肽过氧化物酶(glutathione peroxidases,GPx)(P<0.05)活力均显著高于舍饲饲养,说明放牧饲养羊肉中的抗氧化酶活力较高,能有效抑制脂质氧化。通过抗氧化酶相关调控基因表达量的分析得出,舍饲饲养羊肉中脂肪氧合酶基因表达量高度显著高于放牧饲养(P<0.001),而SOD(P<0.001)、CAT(P<0.05)和GPx(P<0.05)基因表达量均显著低于放牧饲养,从分子水平验证了放牧饲养羊肉的抗氧化能力较舍饲饲养好。

关键词: 饲养方式, 苏尼特羊, 脂质氧化, 抗氧化酶, 基因表达

Abstract: The objective of this study was to investigate the oxidative stability of meat from Sunit sheep fed on two different diets: pasture and forage supplemented with concentrate mixture. The amount of lipid oxidation products, thiobarbituric acid (TBA) value, antioxidant ability, antioxidant enzymes activities and the expression of antioxidant genes in Biceps femoris muscles from sheep slaughtered at 12 months of age were determined and compared between the two feeding groups. The results showed that TBA value of meat from grazed sheep was significantly lower than that of forage plus concentrate-fed sheep (P < 0.001). Hexanal, heptanal, nonanal, 1-octen-3-ol and 2,3-octanedione were the major lipid oxidation products in both meat samples and their values were lower in meat from grazed sheep than in forage plus concentrate-fed sheep (P < 0.05), indicating that the latter had a higher degree of lipid oxidation. As for antioxidant properties, total antioxidant capacity (T-AOC, P < 0.01) and cupric reducing antioxidant capacity (CUPRAC, P < 0.05) of grazed sheep were significantly higher than those of forage plus concentrate-fed sheep. The same was true for the antioxidant enzymes, superoxide dismutase (SOD, P < 0.001), catalase (CAT, P < 0.05) and glutathione peroxidase (GPx, P < 0.05). These observations suggest that meat from pasture-fed sheep has higher antioxidant enzymes activities and as a result, its lipid oxidation can be effectively inhibited. Furthermore, the gene expression of SOD (P < 0.001), CAT (P < 0.05) and GPx (P < 0.05) in pasture-fed sheep was significantly higher than in forage plus concentrate-fed sheep, while the opposite was true for the gene expression of lipoxygenase (P < 0.001). In conclusion, this study provides molecular evidence that antioxidant capacity in meat from pasture-fed sheep was better than in forage plus concentrate-fed sheep.

Key words: feeding pattern, Sunit sheep, lipid oxidation, antioxidant enzymes, gene expression

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