食品科学 ›› 2019, Vol. 40 ›› Issue (22): 199-206.doi: 10.7506/spkx1002-6630-20181126-295

• 生物工程 • 上一篇    下一篇

东北传统发酵食品中降胆固醇乳酸菌的筛选及其降解机制

任大勇,曲天铭,杨柳,安彬,王国超,冯时蓉   

  1. (吉林农业大学食品科学与工程学院,吉林 长春 130118)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    吉林省教育厅“十三五”科学技术项目(JJKH20190926KJ);吉林农业大学大学生科技创新基金项目(2018088)

Screening of Lactic Acid Bacterial Isolates from Traditional Fermented Foods in Northeast China for Cholesterol-Lowering Property and Mechanism of Action Analysis

REN Dayong, QU Tianming, YANG Liu, AN Bin, WANG Guochao, FENG Shirong   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Online:2019-11-25 Published:2019-12-02

摘要: 从中国东北传统发酵食品黏面子、辣白菜、辣酱中筛选具有高效降解胆固醇作用的乳酸菌,并探讨其主要的降解机制。采用体外实验筛选出高效降胆固醇的乳酸菌菌株,进一步研究了其降胆固醇的作用机制,包括细胞膜的吸附、胆汁盐水解酶基因表达及酶活性、胆汁酸共沉淀胆固醇以及抑制胆固醇胶束形成等。筛选得到6 株高胆固醇清除能力的菌株C1、C2、H6、H9、L22和L30,清除率均在85%以上。受试菌株均可以通过膜吸附、共沉淀、胆汁盐水解酶作用和抑制胆固醇胶束等机制发挥作用,其中以胆汁盐水解酶的作用为主要机制。

关键词: 降胆固醇, 乳酸菌, 筛选, 胆汁盐水解酶, 降解机制

Abstract: Lactic acid bacterial isolates derived from three traditional fermented foods in Northeast China, known as NianMianZi, spicy cabbage and chilli sauce were screened for cholesterol-lowering property, and the cholesterol-lowering mechanism was interpreted from the aspects of cell membrane adsorption, bile salt hydrolase gene expression and enzymatic activity, bile acid co-precipitation of cholesterol, cholesterol-inhibiting micelles. Strains C1, C2, H6, H9, L22 and L30 were found to have high cholesterol scavenging ability and their clearance rates were all above 85%. All the strains could effectively exert their cholesterol-lowering effects through bile salt hydrolase as well as membrane adsorption, coprecipitation, and inhibition of cholesterol micelles.

Key words: cholesterol degradation, lactic acid bacteria, screening, bile salt hydrolase, degradation mechanism

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