食品科学 ›› 2019, Vol. 40 ›› Issue (23): 183-188.doi: 10.7506/spkx1002-6630-20181110-120

• 营养卫生 • 上一篇    下一篇

杂粮早餐粉对砷致小鼠雄性生殖毒性的缓解作用

高俊宇,仪慧兰   

  1. (山西大学生命科学学院,山西 太原 030006)
  • 出版日期:2019-12-15 发布日期:2019-12-24
  • 基金资助:
    国家自然科学基金面上项目(31371868);山西省优秀人才科技创新项目(201605D211031); 山西省重点研发计划项目(社会发展)(201703D321026-4)

Antagonistic Effects of Breakfast Cereals on Arsenic-Induced Reproductive Toxicity in Male Mice

GAO Junyu, YI Huilan   

  1. (School of Life Science, Shanxi University, Taiyuan 030006, China)
  • Online:2019-12-15 Published:2019-12-24

摘要: 以昆明种纯系雄性小白鼠为受试动物,检测杂粮早餐粉对砷致小鼠雄性生殖毒性的影响。砷染毒组小鼠饮用含砷蒸馏水(砷质量浓度为10 mg/L),早餐粉干预组小鼠在饮用含砷蒸馏水的同时分别灌胃7.15 g/100 mL和14.30 g/100 mL杂粮早餐粉溶液,每3 d一次。实验周期60 d,期间小鼠自由取水。灌胃结束后检测小鼠睾丸组织的抗氧化指标与组织结构,计算精子总数和精子畸形率。结果表明:通过饮水摄入砷能使小鼠睾丸脏器系数下降,睾丸组织超氧化物歧化酶(superoxide dismutase,SOD)活力和还原型谷胱甘肽(glutathione,GSH)含量下降,H2O2含量升高,丙二醛(malondialdehyde,MDA)含量显著升高(P<0.05),睾丸组织结构出现病理性改变,精子总数降低,精子畸形率升高;用杂粮早餐粉干预后,小鼠睾丸脏器系数升高,睾丸组织SOD活力和还原型GSH含量升高,H2O2和MDA含量降低,睾丸组织结构损伤得到缓解,精子总数升高,精子畸形率下降。上述结果表明,所用杂粮早餐粉能降低砷致雄性生殖毒性,对睾丸结构和功能有一定的保护作用,对雄性动物生殖健康具有积极意义。

关键词: 杂粮早餐粉, 亚砷酸钠, 小鼠, 生殖毒性, 氧化损伤

Abstract: The effects of breakfast cereals on arsenic-induced reproductive toxicity were examined in male Kunming mice. The mice in the model group were administrated with water containing 10 mg/L arsenic from sodium arsenite, while those in the intervention groups were additionally provided with 7.15 or 14.30 g/100 mL of breakfast cereals once every three days for 60 days. All mice had free access to water during the administration period. The antioxidant indexes and histological structure of mouse testicles were examined, and the total number of sperm and the percentage of abnormal sperms were recorded at the end of administration. Our results showed that arsenic intake from drinking water decreased testicular coefficient, sperm quantity, superoxide dismutase (SOD) activity and glutathione (GSH) content in mouse testicles. The contents of H2O2 and malondialdehyde (MDA) in testicular tissue increased significantly in the arsenic exposed group, accompanied with pathological changes as well as a reduction in total sperm number and an elevation in sperm deformity percentage. After intervention with breakfast cereals, the testicular coefficient increased, and so did the SOD activity and GSH content in testis tissue. In addition, lower contents of H2O2 and MDA appeared in the intervention groups; together with the alleviation of testicular damage, the sperm number increased and the sperm malformation ratio decreased. The above results showed the protective effect of cereal breakfast on arsenic-induced reproductive system damage in male mice, suggesting a preventive approach to reduce arsenic toxicity.

Key words: cereals breakfast, sodium arsenite, mice, reproductive toxicity, oxidative damage

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