食品科学 ›› 2019, Vol. 40 ›› Issue (24): 294-299.doi: 10.7506/spkx1002-6630-20180923-236

• 安全检测 • 上一篇    下一篇

纳米磁珠-电化学适配体传感技术检测牛奶中氨苄青霉素

朱俊亚,李芳,赵兰馨,李席席,刘云宏   

  1. (1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.河南省食品原料工程技术研究中心,河南 洛阳 471023)
  • 出版日期:2019-12-25 发布日期:2019-12-24
  • 基金资助:
    国家自然科学基金青年科学基金项目(31501563);河南省科技攻关项目(172102310617)

Magnetic Beads-Based Electrochemical Aptasensor for the Detection of Ampicillin in Milk

ZHU Junya, LI Fang, ZHAO Lanxin, LI Xixi, LIU Yunhong   

  1. (1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; 2. Henan Engineering Technology Research Center of Food Materials, Luoyang 471023, China)
  • Online:2019-12-25 Published:2019-12-24

摘要: 为满足牛奶中氨苄青霉素高效检测的需要,以纳米磁珠为载体,适配体与氨苄青霉素特异性结合为基础,构建氨苄青霉素电化学适配体传感器。采用碳二亚胺交联法制备修饰有氨苄青霉素的磁珠,该磁珠可与待测样中的氨苄青霉素共同竞争反应体系中的适配体和辣根过氧化物酶,随后利用磁性玻碳电极将上述磁珠吸附于电极检测表面进行电化学测定。最佳条件下,该传感器在1.0×10-12~1.0×10-8 mol/L浓度范围内传感器响应电流与氨苄青霉素浓度呈现良好的线性关系,检测限可达1.0×10-12 mol/L。采用该方法测定市售牛奶样品中的氨苄青霉素,精密度和回收率满意。

关键词: 氨苄青霉素, 适配体, 磁珠, 适配体传感器

Abstract: A novel electrochemical aptasensor using magnetic beads, based on the specific binding of aptamer to ampicillin, was developed for the detection of ampicillin in milk. Ampicillin functionalized magnetic beads were prepared and used to compete with ampicillin in samples for binding to its aptamers and horse radish peroxidase. Then a magnetic electrode was used to capture and focus the resulting magnetic beads on its surface. Under the optimal conditions, the net current response was linear with ampicillin concentration in the range of 1.0 × 10-12–1.0 × 10-8 mol/L, with a detection limit of 1.0 × 10-12 mol/L. The method was applied in the determination of ampicillin in milk samples with a satisfactory precision and acceptable recoveries.

Key words: ampicillin, aptamer, magnetic beads, aptasensor

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