食品科学 ›› 2019, Vol. 40 ›› Issue (5): 207-213.doi: 10.7506/spkx1002-6630-20180828-312

• 包装贮运 • 上一篇    下一篇

2,4-表油菜素内酯处理对桃果实软腐病及能量代谢的影响

张正敏,杨艺琳,李美琳,王 静,季娜娜,郑永华*   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2019-03-15 发布日期:2019-04-02
  • 基金资助:
    国家自然科学基金面上项目(31672209);“十三五”国家重点研发计划重点专项(2016YFD0400901)

Effect of 2,4-Epibrassinolide Treatment on Rhizopus Rot and Energy Metabolism in Postharvest Peach Fruit

ZHANG Zhengmin, YANG Yilin, LI Meilin, WANG Jing, JI Nana, ZHENG Yonghua*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2019-03-15 Published:2019-04-02

摘要: 研究了2,4-表油菜素内酯(2,4-epibrassionolide,EBR)处理对‘白凤’桃果实采后软腐病发生及能量代谢的影响。桃果实先用不同浓度(0、1、5、10 μmol/L)EBR溶液浸泡10 min后接种匍枝根霉,后在(20±1)℃下贮藏60 h。结果表明:5 μmol/L的EBR处理能最显著抑制桃果实病斑直径和发病率的上升(P<0.05),同时保持桃果实较好的贮藏品质。与仅接种匍枝根霉的对照组相比,EBR处理能提高Ca2+-ATPase、H+-ATPase、琥珀酸脱氢酶和线粒体细胞色素c氧化酶活力,延缓三磷酸腺苷和二磷酸腺苷含量的下降,抑制一磷酸腺苷含量的上升,从而维持较高的能荷水平;相关性分析显示,对照组(r=-0.939,P<0.01)和EBR处理组(r=-0.962,P<0.01)桃果实的发病率和能荷水平均呈显著负相关。因此,EBR处理抑制桃果实采后软腐病的发生与其维持较高的能荷水平有关。

关键词: 桃果实, 2, 4-表油菜素内酯, 软腐病, 能量代谢

Abstract: The effect of 2,4-epibrassionolide (EBR) treatment on Rhizopus rot and energy metabolism in postharvest peach fruit (Prunus persica (L.) Batsch cv Baifeng) was investigated. Peach fruit were treated with EBR solutions of different concentrations (0, 1, 5 and 10 μmol/L) for 10 min, then inoculated with Rhizopus stolonifer, and finally stored at (20 ± 1) ℃ for 60 hours. The results showed that treatment with EBR at 5 μmol/L was the most effective in suppressing the increase in lesion diameter and disease incidence in peach fruit (P < 0.05) and maintaining good quality during storage. Compared with the control group with the inoculation of Rhizopus stolonifer alone, EBR treatment could increase the activity of Ca2+-ATPase, H+-ATPase, succinate dehydrogenase and cytochrome oxidase, delay the decrease in adenosine triphosphate and adenosine diphosphate contents, and inhibit the increase in adenosine monophosphate content, thus maintaining high energy level of peach fruit. Correlation analysis showed that disease incidence and energy level were significantly negatively correlated in the control (r = ?0.939, P < 0.01) and EBR treatment groups (r = ?0.962, P < 0.01). These results suggested that EBR treatment could inhibit Rhizopus rot of postharvest peach fruit, which may be related to maintenance of high energy level.

Key words: peach fruit, 2,4-epibrassionolide, Rhizopus rot, energy metabolism

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