食品科学 ›› 2020, Vol. 41 ›› Issue (10): 272-280.doi: 10.7506/spkx1002-6630-20190429-381

• 工艺技术 • 上一篇    下一篇

沙棘果油棕榈油酸提取物的富集及改善胰岛素抵抗活性

高山,王嘉佳,胡高爽   

  1. (河北科技大学生物科学与工程学院,河北 石家庄 050018)
  • 出版日期:2020-05-25 发布日期:2020-05-15
  • 基金资助:
    河北科技大学博士科研基金项目(81/1181288;81/1181289);河北科技大学PT基金项目(82/1182218)

Optimizing the Extraction and Enrichment of Plamitoleic Acid from Sea Buckthorn Fruit Oil and Evaluating Its Effect on Alleviating Insulin Resistance

GAO Shan, WANG Jiajia, HU Gaoshuang   

  1. (College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China)
  • Online:2020-05-25 Published:2020-05-15

摘要: 运用尿素包埋-分子蒸馏复合法提取沙棘果油中的棕榈油酸,在单因素试验的基础上采用响应面分析法优化提取工艺参数,并利用气相色谱法对产物脂肪酸组成进行分析。通过高浓度胰岛素诱导HepG2细胞建立胰岛素抵抗模型,探索沙棘果油提取物改善胰岛素抵抗活性。结果表明,尿素包埋和分子蒸馏两步法提取沙棘果油中的棕榈油酸最佳条件为:尿素包埋法,尿素与底物比1.98∶1(g/g)、溶剂与尿素比4.03∶1(mL/g)、包埋时间12.15 h;分子蒸馏法,进料速率0.84 mL/min、刮膜速率116.02 r/min,蒸馏温度99.75 ℃。采用气相色谱检测棕榈油酸含量,结果表明在最佳优化条件下棕榈油酸质量分数显著提高,达到72.19%(模型预测值为68.02%),证明所建立的方法可用于沙棘果油中棕榈油酸的富集和纯化;体外实验表明沙棘果油中棕榈油酸提取物浓度达到100 μmol/L时可以明显改善糖原合成水平,达到200 μmol/L时可以显著性缓解胰岛素抵抗导致的葡萄糖消耗量降低现象,证明沙棘果油棕榈油酸提取物具有改善胰岛素抵抗的功能。

关键词: 沙棘果油棕榈油酸, 提取, 响应面分析, 胰岛素抵抗

Abstract: The extraction of plamitoleic acid (POA) from sea buckthorn fruit oil (SBFO) by urea complexation followed by molecular distillation was optimized using a combination of one-factor-at-a-time method and response surface methodology, and the fatty acid composition of the product was analyzed by gas chromatography (GC). Then, an insulin resistance model was established in HepG2 cells treated with high concentration of insulin and was used to explore the ability of the extract to improve insulin resistance. The results showed that the optimal extraction conditions were as follows: ratio of urea to substrate 1.98:1 (g/g), ratio of solvent to urea 4.03:1 (mL/g), and reaction time 12.15 h for urea complexation; feeding rate 0.84 mL/min, scraping rate 116.02 r/min, and distillation temperature 99.75 ℃ for molecular distillation. The results of GC analysis showed that the plamitoleic acid content was significantly increased up to 72.19% (predicted value of 68.02%) under these optimal conditions, indicating that the established method could be used for the enrichment and purification of POA from SBFO. In vitro experiments showed that the palmitoleic acid at 100 mol/L could significantly improve the level of glycogen synthesis, and alleviate the decrease of glucose consumption caused by insulin resistance at 200 mol/L, proving that a certain dose of sea buckthorn plamitoleic acid could alleviate insulin resistance.

Key words: plamitoleic acid from sea buckthorn fruit oil, extraction, response surface methodology, insulin resistance

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