食品科学 ›› 2020, Vol. 41 ›› Issue (14): 138-145.doi: 10.7506/spkx1002-6630-20190719-247

• 生物工程 • 上一篇    下一篇

清除蜡样芽孢杆菌生物被膜的CIP程序优化

黄子彧,林莹莹,宋思家,任发政,郭慧媛,   

  1. (1.中国农业大学 北京食品营养与人类健康高精尖创新中心,北京 100083;2.中国农业大学 教育部北京市共建功能乳品重点实验室,北京 100083)
  • 发布日期:2020-07-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0400305)

Optimization of Cleaning-in-place Regime for Removal of Bacillus cereus Biofilm

HUANG Ziyu, LIN Yingying, SONG Sijia, REN Fazheng, GUO Huiyuan,   

  1. (1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China; 2. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100083, China)
  • Published:2020-07-29

摘要: 以生物被膜形成能力最强、酸碱耐受性高的菌株A1为对象,首先通过正交试验优化就地清洗程序条件为17.5 g/L氢氧化钠溶液85 ℃冲洗20 min,然后用纯水冲洗,接着用15 g/L硝酸溶液85 ℃冲洗20 min,最后用纯水冲洗。经过此清洗程序后,可以彻底清除不锈钢片上的蜡样芽孢杆菌。为了降低酸碱清洗剂的用量,将酸碱清洗剂与杀菌剂过氧乙酸进行联合使用,优化最佳清洗程序为15.0 g/L氢氧化钠溶液85 ℃冲洗10 min,然后用纯水冲洗,接着用10.0 g/L硝酸溶液85 ℃冲洗10 min,然后使用3 g/L过氧乙酸溶液25 ℃冲洗10 min,最后使用中和剂冲洗。该清洗程序在达到彻底清除蜡样芽孢杆菌的同时,能够降低酸碱清洗剂的浓度,缩短整个清洗程序的时间,为食品加工设备的清洗提供一种更高效、更环保的方法。

关键词: 就地清洗程序;蜡样芽孢杆菌;生物被膜;正交试验;过氧乙酸

Abstract: In this study, using an orthogonal array design, the optimum cleaning-in-place (CIP) regime for thoroughly removing the biofilm of Bacillus cereus A1, which has excellent biofilm-forming ability and high alkali and acid tolerance, on stainless steel sheets was determined as sequential washing with 17.5 g/L NaOH solution at 85 ℃ for 20 min, water, 15 g/L HNO3 at 85 ℃ for 20 min, and water once again. To reduce their use, acid and alkali cleaning agents were used in combination with peracetic acid, and the optimized CIP protocol was determined as sequential washing with 15.0 g/L NaOH solution at 85 ℃ for 10 min, water, 10.0 g/L HNO3 at 85 ℃ for 10 min, 3 g/L peracetic acid at 25 ℃ for 10 min, and neutralizer. This CIP protocol could not only thoroughly remove Bacillus cereu, but also reduce the concentration of acid and alkali cleaning agents and the cleaning time, providing a more efficient and eco-friendly method to clean food processing equipment.

Key words: cleaning-in-place; Bacillus cereus; biofilm; orthogonal array design; peroxyacetic acid

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