食品科学 ›› 2020, Vol. 41 ›› Issue (9): 222-237.doi: 10.7506/spkx1002-6630-20190411-145

• 专题论述 • 上一篇    下一篇

植物多酚氧化酶的生理功能、分离纯化及酶促褐变控制的研究进展

王馨雨,杨绿竹,王婷,王蓉蓉,刘洁,单杨,张群,丁胜华   

  1. (1.湖南大学研究生院隆平分院,湖南 长沙 410125;2.湖南省农业科学院农产品加工研究所,果蔬贮藏加工与质量安全湖南省重点实验室,湖南 长沙 410125;3.吐鲁番市林果业技术推广服务中心,新疆 吐鲁番 838000;4.湖南农业大学食品科学技术学院,湖南 长沙 410128)
  • 出版日期:2020-05-15 发布日期:2020-05-15
  • 基金资助:
    国家自然科学基金青年科学基金项目(31501543);湖南省自然科学基金项目(2016JJ6077); 湖南省农业科技创新项目(2019JG01);湖南省长沙市杰出创新青年培养计划项目(KQ1905025)

Recent Progress toward Understanding the Physiological Function, Purification, and Enzymatic Browning Control of Plant Polyphenol Oxidases

WANG Xinyu, YANG Lüzhu, WANG Ting, WANG Rongrong, LIU Jie, SHAN Yang, ZHANG Qun, DING Shenghua   

  1. (1. Longping Branch, Graduate School of Hunan University, Changsha 410125, China; 2. Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety, Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 3. Turpan Technology Extention Center of Forestry and Fruit Industry, Turpan 838000, China; 4. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China)
  • Online:2020-05-15 Published:2020-05-15

摘要: 多酚氧化酶是一类能催化多酚类物质氧化的含铜质体的金属酶,在植物、动物、微生物中广泛存在。一方面对植物生理功能有重要作用;另一方面也引起植物,主要是果蔬品质的下降,造成严重经济损失。本文系统总结了多酚氧化酶的生理功能、分离纯化、酶学性质、酶促褐变控制方法等研究进展,以期为多酚氧化酶的基础研究与应用研究提供参考依据。

关键词: 多酚氧化酶, 生理功能, 分离纯化, 酶促褐变控制

Abstract: Polyphenol oxidases (PPOs) are a group of metalloenzymes containing copper plastid that can catalyze the oxidation of polyphenols, which widely exist in plants, animals, and microorganisms. On the one hand, they play an important role in the physiological functions of plants; on the other hand, they also cause a decline in fruit and vegetable quality, thereby resulting in a large number of economic losses. In this paper, the physiological function, extraction and purification, and enzymatic properties of PPOs, as well as the different methods for inhibiting PPO acticity for enzymatic browning control are summarized, which will provide a references for basic research and application of PPO.

Key words: polyphenol oxidases, physiological function, purification, enzymatic browning control

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