食品科学 ›› 2010, Vol. 31 ›› Issue (4): 21-23.doi: 10.7506/spkx1002-6300-201004005

• 工艺技术 • 上一篇    下一篇

叶绿体脱除亚硫酸盐效果研究

黄国平,姚玉静,陈黎斌   

  1. 广东省食品药品职业学院
  • 收稿日期:2009-02-24 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 黄国平 E-mail:hgp888@sohu.com
  • 基金资助:

    高等学校博士学科点专项科研基金资助课题(20070561076)

Chloroplast for Removal of Sulfites from Foods

HUANG Guo-ping,YAO Yu-jing,CHEN Li-bin   

  1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China
  • Received:2009-02-24 Online:2010-02-15 Published:2010-12-29
  • Contact: HUANG Guo-ping E-mail:hgp888@sohu.com

摘要:

对从植物中提取的叶绿体应用于脱除食品中亚硫酸盐的效果进行研究,采用单因素试验和响应面分析法确定实验的最优条件。结果表明,叶绿体脱除NaHSO3 的最佳条件为pH8、反应时间3h、叶绿体和NaHSO3 质量比1:250,在此条件下,亚硫酸盐的清除率可达到94.85%。光照、氧气和振荡处理对亚硫酸盐的氧化反应具有促进作用。

关键词: 叶绿体, 脱除, 亚硫酸盐, 响应面分析

Abstract:

Chloroplast extracted from fresh spinach was used for the removal of sulfites from foods in the present study. The optimal technological conditions were investigated using single factor method and central composite design coupled with response surface methodology (RSM). Results showed that the optimal sulfites removal process was based on the reaction between spinach chloroplast and NaHSO3 for 3 h at pH 8 in a proportion of 1:250 (m/m). A removal rate of sulfites of 94.85% was observed using the optimal removal process. In addition, light illumination, oxygen exposure and shake promoted the oxidation of NaHSO3.

Key words: chloroplast, removal, sulfites, response surface design

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