食品科学 ›› 2010, Vol. 31 ›› Issue (4): 59-62.doi: 10.7506/spkx1002-6300-201004013

• 工艺技术 • 上一篇    下一篇

滑菇多糖的超声波辅助提取工艺及抗氧化活性研究

于淑池,侯金鑫,李明艳,李鹏程,陈爱萍   

  1. 湖州师范学院生命科学学院
  • 收稿日期:2009-04-01 修回日期:2009-08-19 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 于淑池 E-mail:yushuchi@126.com
  • 基金资助:

    浙江省2008 年度大学生科技创新项目

Ultrasonic-assisted Extraction and in vitro Antioxidant Evaluation of Pholiota nameko Polysaccharides

YU Shu-chi,HOU Jin-xin,LI Ming-yan,LI Peng-cheng,CHEN Ai-ping   

  1. School of Life Sciences, Huzhou Teachers College, Huzhou 313000, China
  • Received:2009-04-01 Revised:2009-08-19 Online:2010-02-15 Published:2010-12-29
  • Contact: YU Shu-chi E-mail:yushuchi@126.com

摘要:

目的:确定滑菇多糖(Pholiota nameko polysaccharides,PNP)在超声波辅助条件下提取的最佳工艺及体外抗氧化活性。方法:研究浸提温度、超声功率、超声时间、料液比4 个因素对多糖提取率的影响,并通过正交试验确定超声波辅助提取滑菇多糖的最佳工艺;体外抗氧化活性采用在体外化学模拟条件下,测得滑菇多糖的总还原力,以及对超氧阴离子自由基(O2·)、羟自由基(·OH)的清除作用。结果:超声波辅助提取滑菇多糖的最佳工艺参数为浸提温度75℃、超声时间16min、超声功率700W、料液比1:30(g/mL);此条件下的滑菇多糖粗品得率达9.17%;滑菇多糖具有较强的还原力和较强的自由基清除作用。结论:滑菇多糖可作为潜在天然抗氧化剂应用于食品和医药工业中。

关键词: 滑菇, 多糖, 超声波, 正交试验, 抗氧化作用

Abstract:

Fruit bodies of Pholiota nameko were used as the raw material to extract Pholiota nameko polysaccharide (PNP) by ultrasonic-assisted extraction. Optimal PNP extraction process was investigated using orthogonal array design. To evaluate the antioxidant activity of PNP, total reducing power and scavenging capacity against superoxide anion or hydroxyl radicals were assayed using in vitro simulated chemical systems. Results showed that the optimum PNP extraction conditions were as follows: ultrasonic power 700 W, ultrasonic treatment time 16 min, extraction temperature 75 ℃, and solid/liquid ratio 1:30. Under the optimum conditions, PNP yield reached up to 9.17%. PNP showed strong in vitro reducing power and free radical-scavenging capacity. Thus, PNP has good potential as a natural antioxidant used in food or medicine industries.

Key words: Pholiota nameko, polysaccharide, ultrasonic, orthogonal array design, antioxidant activity

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