食品科学 ›› 2010, Vol. 31 ›› Issue (4): 90-94.doi: 10.7506/spkx1002-6300-201004020

• 工艺技术 • 上一篇    下一篇

富硒发芽糙米生产工艺的优化

江 湖1,付金衡1, 2,*,苏 虎3,余 勃1,欧阳武军1,方蓝堃1   

  1. 1.南昌大学中德联合研究院,食品科学与技术国家重点实验室 2.江西中德创业有限公司 3.江西科技师范学院生命科学学院
  • 收稿日期:2009-03-19 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 付金衡 E-mail:fujinheng@ncu.edu.cn

Response Surface Methodology for Optimization of Germination of Selenium-enriched Brown Rice

JIANG Hu1,FU Jin-heng1,2,*,SU Hu3,YU Bo1,OUYANG Wu-jun1,FANG Lan-kun1   

  1. 1. Sino-German Joint Research Institute, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang
    330047, China;2. Jiangxi Zhongde Chuangye Co. Ltd., Nanchang 330047, China;3. College of Life Science, Jiangxi Science and
    Technology Normal University, Nanchang 330013, China
  • Received:2009-03-19 Online:2010-02-15 Published:2010-12-29
  • Contact: FU Jin-heng E-mail:fujinheng@ncu.edu.cn

摘要:

目的:研究富硒发芽糙米的最佳生产工艺。方法:通过单因素试验及Box-Behnken 组合设计考察浸泡液中亚硒酸钠质量浓度、发芽时间、发芽温度以及它们之间的交互作用对糙米发芽率及重要营养物质γ- 氨基丁酸含量的影响,通过软件分析得到使发芽率及γ- 氨基丁酸含量均达到最大值的最佳生产工艺。结果:建立富硒发芽糙米发芽率与γ- 氨基丁酸含量的数学模型,富硒发芽糙米的最佳工艺参数为:浸泡液中亚硒酸钠质量浓度5mg/L,浸泡时间9h,浸泡温度28℃,培养时间21h,培养温度31.5℃。该条件下得到发芽率77.67%,GABA 含量330mg/kg,硒含量0.5mg/kg 的富硒发芽糙米。

关键词: 发芽糙米, 富硒, 发芽率, γ- 氨基丁酸, Box-Behnken 组合设计

Abstract:

To obtain both optimal germination rate and γ-aminobutyric acid content, germination conditions such as sodium selenite concentration in soaking solution, germination time and germination temperature were investigated using Box-Behnken central composite design (CCD) combined with response surface methodology. Box-Behnken CCD experimental data were fitted to establish a mathematical model for germination rate or γ-aminobutyric acid content as a function of the above variables. Results showed that the optimal production process of Se-enriched germinated brown rice was achieved by soaking for 9 h in 5 mg/L sodium selenite solution at 28 ℃ followed by cultivation for 21 h at 31.5℃. Under these conditions, germination rate was 77.67% and the contents of γ-aminobutyric acid and Se in germinated brown rice were 330 mg/kg and 0.5 mg/kg, respectively.

Key words: germinated brown rice, selenium enrichment, germination rate, &gamma, -aminobutyric acid, Box-Behnken design

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