食品科学 ›› 2010, Vol. 31 ›› Issue (4): 236-238.doi: 10.7506/spkx1002-6300-201004055

• 分析检测 • 上一篇    下一篇

反相高效液相色谱法测定肉果秤锤树叶中的熊果酸

刘素君,周泽斌,唐 梅   

  1. 乐山师范学院化学与生命科学学院
  • 收稿日期:2009-04-16 修回日期:2009-09-16 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 刘素君 E-mail:zouliurui@126.com
  • 基金资助:

    乐山师范学院青年教师基金项目(Z07033)

HPLC Determination of Ursolic Acid in Leaves of Sinojackia sarcocarpa L.Q.Luo

LIU Su-jun,ZHOU Ze-bin,TANG Mei   

  1. Department of Chemistry and Life Sciences, Leshan Normal University, Leshan 614004, China
  • Received:2009-04-16 Revised:2009-09-16 Online:2010-02-15 Published:2010-12-29
  • Contact: LIU Su-jun E-mail:zouliurui@126.com

摘要:

目的:建立肉果秤锤树树叶中熊果酸含量的高效液相色谱测定方法。方法:采用Shim-pack VP-ODS C18柱(150mm × 4.6mm,5μm),甲醇- 水- 磷酸(850:147:3,V/V)为流动相,检测波长210nm,流速1.0mL/min。结果:熊果酸的进样量在0.124~2.48μg/μL 范围内与峰面积积分值具有良好的线性关系(R2 = 0.9971),平均回收率为100.5%,RSD 为0.91%。超声提取的熊果酸含量最高。结论:该方法稳定性、重现性好,且简单易行。

关键词: 反相高效液相色谱法, 秤锤树, 熊果酸

Abstract:

A HPLC method was developed for the determination of ursolic acid in leaves of Sinojackia sarcocarpa L.Q.Luo. The chromatographic separation was achieved on a Shim-pack VP-ODS C18 column using a mobile phase composed of H2O and H3PO4 (850:147:3, V/V) at a flow rate of 1 mL/min, and the detection wavelength was set at 210 nm. A good linearity of the standard curve for peak area versus ursolic acid concentration was obtained over the range of 0.124-2.48μg/μL (R2 = 0.9971). The average spike recovery of ursolic acid at three levels was 100.5% (n = 3), with 0.91% RSD. In sample preparation, ultrasonic extraction resulted in higher efficiency when compared to reflux extraction and percolation extraction. This method reveals the characteristics of good stability and repeatability, simplicity, and easy operation.

Key words: HPLC, Sinojackia sarcocarpa, ursolic acid

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