食品科学 ›› 2010, Vol. 31 ›› Issue (4): 250-253.doi: 10.7506/spkx1002-6300-201004059

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定奶粉中的三聚氰胺及其不确定度分析

杨 洋,徐春祥,车文军   

  1. 江苏省产品质量监督检验研究院
  • 收稿日期:2009-03-31 修回日期:2009-09-04 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 杨洋 E-mail:yy2304@126.com

Uncertainty Evaluation of HPLC Determination of Melamine in Milk Powderchromatography

YANG Yang,XU Chun-xiang,CHE Wen-jun   

  1. Jiangsu Provincial Supervising and Testing Institute for Products Quality, Nanjing 210029, China
  • Received:2009-03-31 Revised:2009-09-04 Online:2010-02-15 Published:2010-12-29
  • Contact: YANG Yang E-mail:yy2304@126.com

摘要:

建立高效液相色谱法测定奶粉中三聚氰胺结果不确定度评定的数学模型,全面考虑测量过程中的不确定度来源,分别运用最小二乘法对标准曲线拟合的不确定度,以及极差法对测定次数较少时引起的不确定度进行评定。结果表明,取样量2.00mg 时,测定奶粉中三聚氰胺含量为(2.00 ± 0.088)mg/kg。本方法适用于高效液相色谱法测定奶粉中三聚氰胺的不确定度评定。

关键词: 不确定度, 高效液相色谱, 三聚氰胺, 奶粉

Abstract:

The sources of uncertainty in high performance liquid chromatographic (HPLC) determination of melamine in milk powder were fully analyzed according to the calculation formula of melamine. Each uncertainty component was modeled. Uncertainty evaluation was conducted for fitting of standard curve of melamine and less measurement times by least square method and extreme deviation method, respectively. HPLC determination with uncertainty evaluation by the method gave the (2.00 ± 0.088) mg/kg melamine content in a randomly inspected commercial milk powder (weighed 2.00 mg). This method proves to be applicable to the uncertainty evaluation of HPLC determination of melamine in milk powder.

Key words: uncertainty, HPLC, melamine, milk powder

中图分类号: