食品科学 ›› 2010, Vol. 31 ›› Issue (1): 14-18.doi: 10.7506/spkx1002-6300-201001003

• 基础研究 • 上一篇    下一篇

荔枝皮原花青素的体外抗氧化活性和对DNA损伤的保护作用

李书艺,周玮婧,孙智达* ,谢笔钧   

  1. 华中农业大学食品科学技术学院
  • 收稿日期:2009-03-24 修回日期:2009-08-19 出版日期:2010-01-01 发布日期:2014-05-19
  • 通讯作者: 孙智达 E-mail:sunzhida@mail.hzau.edu.cn
  • 基金资助:

    国家自然科学基金- 广东省联合基金项目(U0731005)

Antioxidant Activity and DNA Damage Preventing Effect of Procyanidins from Litchi chinensis Pericarp

LI Shu-yi,ZHOU Wei-jing,SUN Zhi-da*,XIE Bi-jun   

  1. (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Received:2009-03-24 Revised:2009-08-19 Online:2010-01-01 Published:2014-05-19
  • Contact: SUN Zhi-da*, E-mail:sunzhida@mail.hzau.edu.cn

摘要:

分别采用邻苯三酚- 鲁米诺化学发光体系、硫酸亚铁- 鲁米诺- 过氧化氢化学发光体系和过氧化氢- 鲁米诺化学发光体系测定白腊、妃子笑、糯米糍和怀枝4 个品种的荔枝皮原花青素(procyanidins of litchi pericarp,LPPC)体外清除超氧阴离子自由基、羟自由基和过氧化氢的能力。同时运用硫酸铜- 邻菲啰啉- 抗坏血酸- 过氧化氢-DNA体系测定不同LPPC 对诱导体系DNA 损伤的保护作用。实验结果表明:白腊LPPC 具有较好的体外清除超氧阴离子自由基和过氧化氢的能力;妃子笑呈现出较显著的羟自由基清除能力和保护DNA 损伤的效果。不同品种的LPPC在不同化学发光体系中的抗氧化活性呈现较大差异。

关键词: 荔枝皮原花青素, 化学发光法, 体外抗氧化活性, 保护DNA 损伤

Abstract:

In order to evaluate antioxidant activity in vitro of procyanidins from pericarp of four cultivars (Baila, Feizixiao, Nuomizi, Huaizhi) of litchi (LPPC), superoxide anion radical, hydroxyl radical and hydrogen peroxide scavenging assays were carried out using pyrogallo-luminol, ferrous sulfate-Luminol-hydrogen peroxide and Luminol-hydrogen peroxide systems, respectively. In addition, copper sulfate-phen-VC-hydrogen peroxide-DNA system was used to determine the preventing effect of LPPC against DNA damage. Results showed that among the litchi cultivars, Baila LPPC possessed the best scavenging potency against superoxide anion and hydrogen peroxide and Feizixiao LPPC indicated the most efficient hydroxide radical scavenging ability and DNA damage preventing effect. In conclusion, procyanidins from pericarp of different litchi cultivars acted different antioxidant effects in different chemiluminescence systems.

Key words: LPPC, chemiluminescence method, antioxidant activity in vitro, DNA damage prevention

中图分类号: