食品科学 ›› 2010, Vol. 31 ›› Issue (1): 91-94.doi: 10.7506/spkx1002-6630-201001021

• 基础研究 • 上一篇    下一篇

夏枯草水溶性酸性多糖的分离及活性分析

魏 明,熊双丽* ,金 虹,徐国波,夏 翠   

  1. 西南科技大学生命科学与工程学院
  • 收稿日期:2009-04-07 出版日期:2010-01-01 发布日期:2014-05-19
  • 通讯作者: 熊双丽 E-mail:xiongshuangli@swust.edu.cn
  • 基金资助:

    西南科技大学国防重点学科实验室重点培育项目(07JGZB18)

Isolation and Antioxidant Activity of Water-soluble Acidic Polysaccharide from Prunella vulgaris Linn

WEI Ming,XIONG Shuang-li*,JIN Hong,XU Guo-bo,XIA Cui   

  1. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
  • Received:2009-04-07 Online:2010-01-01 Published:2014-05-19
  • Contact: XIONG Shuang-li*, E-mail:xiongshuangli@swust.edu.cn

摘要:

采用水提醇沉法提取夏枯草多糖,然后经非极性吸附树脂HZ-820 脱色和脱蛋白后,再用DEAE-SepharoseFast Flow 阴离子交换柱层析进行分离得到酸性多糖。化学法和红外光谱证明该酸性多糖中半乳糖醛酸和硫酸根含量较高,间羟基联苯- 硫酸和氯化钡- 明胶比浊法测定其半乳糖醛酸和硫酸根含量分别为(58.85 ± 0.46)% 和(7 ± 0.52)%。紫外、红外光谱分析结果发现,该酸性杂多糖为非糖蛋白,可能含半乳糖醛酸、木糖、葡萄糖、鼠李糖和半乳糖等单糖。抗氧化活性研究表明该酸性多糖具有显著的DPPH 自由基和羟自由基清除活性。

关键词: 夏枯草, 酸性多糖, 分离纯化, 抗氧化

Abstract:

An acidic polysaccharide with antioxidant activity was obtained from Prunella vulgaris Linn through sequential steps of water extraction and alcohol precipitation, then decolorization and deproteinization by HZ-820-type unpolar resin adsorption, and finally purification. 3-Hydroxydiphenyl-sulfuric acid and barium chloride-gelatin turbidimetric methods were used to determine contents of galacturonic acid and sulfate in the polysaccharide, and the results were (58.85 ± 0.46)% and (7 ± 0.52)%, respectively. Ultraviolet and infrared (IR) spectral analyses indicated that the monosaccharide composition of the polysaccharide consisted of galacturonic acid, xylose, glucose, rhamnose, and galactose and it belonged to the nonglycoprotein family. Moreover, this polysaccharide revealed strong scavenging activities against hydroxyl free radicals and DPPH free radicals.

Key words: Prunella vulgaris Linn, acidic polysaccharide, isolation and purification, antioxidation

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