关键词: 香菇, SSR, EST, 引物

Abstract:

Simple sequence repeats (SSRs) were investigated in 12144 qualified ESTs downloaded from Nucleotide ESTs database of Lentinula edodes in NCBI. There were 359 SSRs in all the ESTs sequences of Lentinus edode, which were composed of 243 di- (67.69%), 114 tri- (31.76%), 1 penta- (0.28%) and 1 hexa- (0.28%) nucleotide SSRs. The estimated frequency of SSRs was approximately 1 SSR per 33.8 ESTs. Total length of SSRs was 7727 kb, accounting for about 2.94% of total ESTs sequences. In addition, we selected 100 ESTs sequences in Lentinus edodes and designed 100 primer pairs for EST-SSR markers, of which 20 were arbitrarily selected and synthesized. Selection and investigation of these primers were performed with 4 wild-type strains of Lentinula edodes. There were 16 primer pairs characterized by clear and stable target bands, of which 11 presented genetic diversity. Our findings indicated that these Lentinula edodes EST-SSRs markers developed in this study were useful and effective.

Key words: Lentinus edodes, SSR, EST, primers