关键词: 天麻糖蛋白, 快速蛋白液相色谱, 急性血瘀, 血液流变

Abstract:

Objective: To isolate and purify glycoprotein from Gastrodia elata (GGE2b) and investigate its hemorrheological effect in acute blood stasis rats. Methods: Soluble extracts of Gastrodia elata were purified by DEAE-Sepharose column chromatography and fast protein liquid chromatography (FPLC) to obtain GGE2b. Acute blood stasis rat model was developed by hypodermic adrenaline injection combined with cold-shock treatment. Totally 60 male rats were randomly divided into 6 groups of 10 rats each, namely saline group, acute blood stasis model group, PGE2b administration groups at doses of 30, 60 and 120 mg/kg, and positive control group administered with Danshen injection. Effect of GGE2b on whole-blood viscosity, plasma viscosity, red blood cell (RBC) accumulation, RBC aggregation index and RBC deformability index in acute blood stasis rats was examined. Results: GGE2b was successfully purified from Gastrodia elata through combined technology of DEAE-Sepharose column chromatography and FPLC. Moreover, GGE2b administration at the doses of 60 mg/kg and 120 mg/kg in rats resulted in a dramatic decrease of plasma viscosity (P ＜ 0.05), whole-blood viscosity (P ＜0.01) and RBC aggregation index (P＜ 0.05), and increase of RBC deformability index (P ＜0.05). Conclusion: It is feasible to purify glycoproteins from Gastrodia elata using chromatography technology. GGE2b can result in a significant improvement of abnormal hemorrheology in rats with acute blood stasis.

Key words: glycoprotein from Gastrodia elata, fast protein liquid chromatography, acute blood stasis, hemorrheology