食品科学 ›› 2010, Vol. 31 ›› Issue (3): 114-117.doi: 10.7506/spkx1002-6300-201003026

• 基础研究 • 上一篇    下一篇

碳氮比对Phaffia rhodozyma CS0217 虾青素合成的影响

梅艳珍,王立梅,郑丽雪,齐 斌*   

  1. 常熟理工学院生物与食品工程学院
  • 收稿日期:2009-03-31 出版日期:2010-02-01 发布日期:2010-12-29
  • 通讯作者: 齐斌 E-mail:qibin65@126.com

Effect of C/N Ratio on Astaxanthin Synthesis in Yeast Phaffia rhodozyma CS0217

MEI Yan-zhen,WANG Li-mei,ZHENG Li-xue,QI Bin*   

  1. Faculty of Food and Bioengineering, Changshu Institute of Technology, Suzhou 215500, China
  • Received:2009-03-31 Online:2010-02-01 Published:2010-12-29
  • Contact: QI Bin E-mail:qibin65@126.com

摘要:

碳氮比(C/N)对Phaffia rhodozyma CS0217 虾青素的合成有重要影响,摇瓶实验结果表明:随着C/N 的降低,虾青素含量显著降低,当C/N 为4.5:0.1 时,每克干细胞虾青素的最高含量为2.48mg/g;在高C/N 条件下酵母发芽率低,控制酵母发芽率小于0.5,有利于虾青素的合成;进一步通过添加放线菌酮发现,由β - 胡萝卜素向虾青素转化的关键酶可能在细胞培养的对数生长期受到抑制。

关键词: 虾青素, Phaffia rhodozyma CS0217, 碳氮比

Abstract:

Astaxanthin synthesis in yeast Phaffia rhodozyma CS0217 was greatly dependent on C/N ratio. The amount of synthesized astaxanthin exhibited a dramatic decrease due to the decrease of C/N ratio. The highest astaxanthin productivity was 2.48 mg/g at the C/N ratio of 4.5:0.1. High C/N ratio could result in a low budding rate of yeast, which was benefit for astaxanthin synthesis. The optimal budding rate of yeast should be controlled below 0.5. Furthermore, the addition of cycloheximide in the culture medium for yeast could inhibit the activity of critical enzymes that involved in the conversion from β -carotene to astaxanthin.

Key words: astaxanthin, Phaffia rhodozyma CS0217, C/N ratio

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