水产品中副溶血性弧菌的污染、毒力基因及耐药性研究

doi:10.7506/spkx1002-6300-201003048

食品科学 ›› 2010, Vol. 31 ›› Issue (3): 209-212.doi: 10.7506/spkx1002-6300-201003048

水产品中副溶血性弧菌的污染、毒力基因及耐药性研究

安秀华，宁喜斌*，李涛

上海海洋大学食品学院

收稿日期:2009-04-27 修回日期:2009-09-26 出版日期:2010-02-01 发布日期:2010-12-29
通讯作者: 宁喜斌 E-mail:anxiuhua2005@yahoo.com.cn

Contamination, Virulence Genes and Drug Resistance of Vibrio parahaemolyticus from Aquatic Products

AN Xiu-hua NING Xi-bin* LI Tao

College of Food Science and Technology, Shanghai Ocean University Shanghai 201306, China

Received:2009-04-27 Revised:2009-09-26 Online:2010-02-01 Published:2010-12-29
Contact: NING Xi-bin E-mail:anxiuhua2005@yahoo.com.cn

摘要/Abstract

摘要：

采集上海市各大农贸市场3类水产品共273份，分离出副溶血性弧菌105株，平均检出率为38.46%，其中甲壳类、贝类、鱼类检出率分别为50.96%、27.12%、15.79%，三者间有极显著差异(P＜0.01)。多重PCR检测分离菌株发现所有分离菌株没有耐热直接溶血毒素和相对耐热直接溶血毒素基因，仅有不耐热溶血毒素基因。K-B氏药敏纸片法检测了105株分离菌株对10种抗生素的耐药性，发现所有菌株对头孢曲松、萘啶酸和诺氟沙星敏感，部分菌株对氨苄西林(69.50%)和阿莫西林(12.38%)等具有较强的耐药性。另外采用碱裂解法提取所有分离菌株的质粒，发现只有7株菌株含有1～3个质粒，大小范围在1～24kb之间，且菌株耐药性与其所携带的质粒的数量和大小并无直接联系。表明分离菌株的耐药性主要是由于细菌染色体相关基因突变造成的。

关键词: 水产品, 副溶血性弧菌(Vp), 毒力基因, 耐药性, 质粒

Abstract:

Added together, 105 strains of Vibrio parahaemolyticus (Vp) were isolated from 273 aquatic products in three species collected from farmers’ markets in Shanghai. The average Vp contamination rate of aquatic products was 38.46%. In addition, the Vp contamination rates of crustacean, shellfish and fish were 50.96%, 27.12% and 15.79%, respectively. A significant difference was observed in three species of aquatic products (P＜0.01). Only tlh gene was detected in isolated Vp strains through multiplex-PCR, while no tdh gene and trh gene were detected. These isolated Vp strains exhibited strong resistance to 10 kinds of antibiotics including ceftriaxone, nalidixic acid and norfloxacin according the evaluation by K-B method. Some of these isolated Vp strains exhibited 69.50% resistance to ampicillin and 12.38% resistance to amoxicillin. Moreover, the analysis of plasmid DNA from 105 isolated strains revealed that seven isolated strains harbored 1－3 plasmids with size ranging from 1 to 24 kb. In analyzing fingerprinting images of plasmids and drug resistant types, no direct relationship between drug resistance and plasmid size or amount was observed. These results suggest that the drug resistance to these antibiotics mainly results from chromosomal mutation of Vp strains.

Key words: aquatic product, Vibrio parahaemolyticus (Vp), virulence gene, drug resistance, plasmid

中图分类号:

R378.3

安秀华，宁喜斌*，李涛. 水产品中副溶血性弧菌的污染、毒力基因及耐药性研究[J]. 食品科学, 2010, 31(3): 209-212.

AN Xiu-hua NING Xi-bin* LI Tao. Contamination, Virulence Genes and Drug Resistance of Vibrio parahaemolyticus from Aquatic Products[J]. FOOD SCIENCE, 2010, 31(3): 209-212.

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水产品中副溶血性弧菌的污染、毒力基因及耐药性研究

Contamination, Virulence Genes and Drug Resistance of Vibrio parahaemolyticus from Aquatic Products

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