食品科学 ›› 2010, Vol. 31 ›› Issue (2): 224-227.doi: 10.7506/spkx1002-6300-201002057

• 分析检测 • 上一篇    下一篇

反相高效液相色谱法测定雪莲果叶中的绿原酸

陈红惠1,2,彭光华3   

  1. 1.华中农业大学食品科学技术学院 2.文山学院生化系 3.西藏自治区质量技术监督局
  • 收稿日期:2009-02-24 出版日期:2010-01-15 发布日期:2010-12-29
  • 通讯作者: 陈红惠 E-mail:suplgrl@163.com

Reversed-Phase High Performance Liquid Chromatographic Determination of Chlorogenic Acid in Yacon Leaves

CHEN Hong-hui1,2,PENG Guang-hua3   

  1. 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Department of Bio-chemistry, Wenshan University, Wenshan 663000, China ;
    3. Tibet Bureau of Quality and Technical Supervision, Lhasa 540000, China
  • Received:2009-02-24 Online:2010-01-15 Published:2010-12-29
  • Contact: CHEN Hong-hui1,2 E-mail:suplgrl@163.com

摘要:

采用反相高效液相色谱法对雪莲果叶中的有效成分绿原酸进行分离,并对其含量进行测定,建立反相高效液相色谱法测定雪莲果叶中绿原酸含量的方法。色谱柱为Hypersil ODS-2 C18 柱(4.6mm × 300mm,5μm),流动相为乙腈- 体积分数0.5% 磷酸溶液(8:92,V/V),流速为1.0mL/min,检测波长为325nm,柱温为25℃。绿原酸在10~100μg/mL 范围内呈良好线性关系,相关系数为0.9999,加标回收率为97.0%,RSD 为1.10%。本法快速、简单、准确、分离度好,可用于实际样品的检测。

关键词: 雪莲果叶, 绿原酸, 反相高效液相色谱法

Abstract:

A method using reversed-phase high performance liquid chromatography was developed for the determination of chlorogenic acid in yacon leaves, an active substance. The chromatographic separation was achieved on a Hypersil ODS column (4.6 mm × 300 mm, 5 μm) using a mobile phase composed of acetonitrile and 0.5% phosphoric acid (8:92, V/V) at a flow rate of 1.0 mL/min. The detection wavelength and the column temperature were set at 325 nm and 25 ℃, respectively. There was a good linear relationship between peak area and chlorogenic acid concentration over the range of 10-100 μg/mL (r = 0.9999), and the average spike recovery for chlorogenic acid was 97.0% with a relative standard deviation (RSD) of 1.10%. The developed method presents the benefits of rapidity, simplicity, high accuracy and good separation.

Key words: yacon leaves, chlorogenic acid, reversed-phase high performance liquid chromatography (RP-HPLC)

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