食品科学 ›› 2009, Vol. 30 ›› Issue (24): 163-165.doi: 10.7506/spkx1002-6630-200924034

• 工艺技术 • 上一篇    下一篇

超声波辅助反胶束提取菜籽蛋白及因素筛选

王立峰1,2,朱均旺1,袁 建1,李 静1,何 荣1,2,鞠兴荣1,*   

  1. 1.南京财经大学食品科学与工程学院,江苏省粮油品质控制及深加工技术重点实验室 2.江南大学食品学院
  • 收稿日期:2009-05-27 修回日期:2009-09-03 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 鞠兴荣1,* E-mail:xingrongju@163.com
  • 基金资助:

    国家“863”计划项目(2007AA10Z331);江苏省教育厅自然科学研究项目(08KJB55004);
    南京财经大学重点研究项目(A0706)

Sonication Assisted Extraction of Rapeseed Protein from Rapeseed Meal in a Reverse Micelle System

WANG Li-feng1,2,ZHU Jun-wang1,YUAN Jian1,LI Jing1,HE Rong1,2,JU Xing-rong1,*   

  1. 1. Key Laboratory of Grain and Oils Quality Control and Deep-Utilizing Technology of Jiangsu Province, College of Food Science and
    Engineering, Nanjing University of Finance and Economics, Nanjing 210003, China;2. School of Food Science and Technology,
    Jiangnan University, Wuxi 214122, China
  • Received:2009-05-27 Revised:2009-09-03 Online:2009-12-15 Published:2010-12-29
  • Contact: JU Xing-rong1,* E-mail:xingrongju@163.com

摘要:

研究超声波辅助反胶束(气溶胶OT/ 异辛烷)提取菜籽蛋白,采用田口设计对影响超声波辅助反胶束提取菜籽蛋白的因素进行了筛选,所选取的6 个相关因素为pH 值、钾离子浓度(mol/L)、提取温度(℃)、提取时间(min)、气溶胶OT(丁二酸二异辛酯磺酸钠)质量(g)、非极性溶剂中的水的浓度和表面活性剂的浓度之比W0,通过对二次多项回归方程求解,获得最优一次拟合多元回归模型方程(P=0.05)为:Y=0.063X1+0.017X2+0.024X4+0.529,筛选出影响超声波辅助提取菜籽蛋白的主要因素:气溶胶OT 质量、pH 值、W0 和提取时间,其中前三者为影响极显著因素。

关键词: 菜籽蛋白, 反胶束, 超声辅助萃取, 因素筛选

Abstract:

Assisted with sonication, rapeseed protein was extracted from rapeseed meal in a reverse micelle system. The effects of the W0, extraction time, pH value, K+ concentration, temperature, and the amount of OT on the extraction rate were studied. A Taguchi experimental design in JMP involving the above 6 factors at 3 levels was employed to optimize the extraction process. Results showed that the best fit regression equation is Y=0.063X1+0.017X2+ 0.024X4+0.529, and the amount of OT, pH value, W0 and extraction time were significant factors that influenced the extraction rate of rapeseed protein.

Key words: rapeseed protein, reverse micelle, sonication assisted extraction, screening factor

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