食品科学 ›› 2009, Vol. 30 ›› Issue (24): 93-98.doi: 10.7506/spkx1002-6630-200924018

• 工艺技术 • 上一篇    下一篇

枸杞多糖碱性乙醇提取及消除羟基自由基活性研究

胡仲秋1,王 利2,王保玲1,王 婧1,岳田利1   

  1. 1.西北农林科技大学食品科学与工程学院 2.西藏职业技术学院
  • 收稿日期:2009-01-21 修回日期:2009-08-21 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 胡仲秋 E-mail:hutiger-2005@126.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAK02A24)

Antioxidant Activity of Polysaccharides from Lycium barbarum

HU Zhong-qiu1,WANG Li2,WANG Bao-ling1,WANG Jing1,YUE Tian-li1   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
    2. Tibet City College of Vocational Technology, Lhasa 850000, China
  • Received:2009-01-21 Revised:2009-08-21 Online:2009-12-15 Published:2010-12-29
  • Contact: HU Zhong-qiu1, E-mail:hutiger-2005@126.com

摘要:

以宁夏枸杞为原料,采用碱性乙醇溶液提取法,通过单因素试验和正交试验,以多糖得率为衡量指标,优选出碱性乙醇提取枸杞多糖的最佳工艺条件,考察条件枸杞多糖消除羟自由基的活性。结果表明:液料比、乙醇体积分数、pH 值、浸提温度、浸提时间对枸杞多糖得率均有显著影响;碱性乙醇提取枸杞多糖最佳条件为液料比50:1(ml/g)、乙醇体积分数8%、pH10.5,于70℃条件下浸提6.5h,枸杞多糖得率最高可达29.19%,比传统水提法提高9.51%;当枸杞多糖质量浓度为2.35mg/ml 时,对羟自由基的消除率可达44.90%。

关键词: 枸杞多糖, 碱性乙醇提取, 优化, 自由基清除活性

Abstract:

An alkaline-ethanol extraction technique was developed to extract LBP and the extraction condition was optimized by a single factor test and orthogonal experiments. The in vitro radical scavenging activity of LBP against ·OH was also discussed. Results indicated that the yield of LBP was significantly affected by the iquid/Solid ratio, ethanol concentration, pH, extraction temperature and extraction time. The optimum conditions for alkali-ethanol extraction were: liquid/solid ratio of 50:1 (ml/g), ethanol concentration of 8%, pH 10.5, 70 ℃ and 6.5 h of extraction time. Under this condition, the yield of LBP could reach the maximum of 29.19%, which was increased by 9.51% compared to the traditional water extraction technology. The radical scavenging activity of LBP samples against ·OH was up to 44.90% at 2.35 mg/ml.

Key words: Lycium barbarum polysaccharids (LBP), alkaline-ethanol extraction technology, optimize, radical scavenging activity

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