鱼降压肽的大孔吸附树脂脱盐及理化性质

doi:10.7506/spkx1002-6630-200924033

食品科学 ›› 2009, Vol. 30 ›› Issue (24): 158-162.doi: 10.7506/spkx1002-6630-200924033

鱼降压肽的大孔吸附树脂脱盐及理化性质

陈季旺¹，孙勤¹，夏文水^1,2

1.武汉工业学院食品科学与工程学院 2.江南大学食品学院

收稿日期:2009-06-02 修回日期:2009-09-28 出版日期:2009-12-15 发布日期:2010-12-29
通讯作者: 陈季旺 E-mail:jiwangchen@yahoo.com.cn
基金资助:
国家“863”计划项目(2006AA09Z444；2007AA091802)；
广东省-科技部产学研结合项目(2007A090302059)；农业部现代农业产业技术体系建设专项资金资助项目(nycytx-49)

Desalting and Physico-chemical Properties of Antihypertensive Peptides from Grass Carp Protein with Macroporous Adsorption Resin

CHEN Ji-wang1，SUN Qin1，XIA Wen-shui1,2

1. School of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China；
2. School of Food Science and Technology, Jiangnan Unviersity, Wuxi 214122, China

Received:2009-06-02 Revised:2009-09-28 Online:2009-12-15 Published:2010-12-29
Contact: CHEN Ji-wang1 E-mail:jiwangchen@yahoo.com.cn

摘要/Abstract

摘要：

研究DA201-C 型大孔吸附树脂对鱼降压肽的脱盐工艺，并分析脱盐后的鱼降压肽的理化性质。结果表明，DA201-C 型大孔吸附树脂较佳脱盐工艺条件为上样质量浓度10mg/ml、上样流速1.5BV/h、解吸剂为70%乙醇。鱼降压肽经DA201-C 型大孔吸附树脂处理后其灰分由13.89% 下降到3.89%，血管紧张素转化酶(ACE)抑制活性由64.35%增加到95.00%。脱盐后的鱼降压肽的分子质量分布范围在200～7000D 之间，其中在200～2000D 之间的肽含量为81.7%；其在pH2～12 的溶解度均为98% 以上，吸湿性跟大豆肽类似，吸湿率比尿素低，比甘油高。

关键词: 鱼降压肽, 大孔吸附树脂, 血管紧张素转化酶(ACE)抑制活性, 脱盐, 理化性质

Abstract:

A certain amount of salts were introduced into fish antihypertensive peptides during the process of fish protein hydrolysis and 10% of ash in the final products was capable of influencing its activities and quality. The desalting technology of fish antihypertensive peptides by DA201-C macroporous adsorption resin was investigated as well as its physic-chemical properties after desalting. The results indicated that the optimum condition for desalting was obtained as follows: loading sample concentration of 10 mg/ml, sampling flow rate of 1.5 BV/h and 70% alcohol as eluent. Under this condition, the ash content in fish antihypertensive peptides was decreased from 13.89% to 3.89%, and its ACE inhibitory activity increased from 64.35% to 95.00%. Molecular weight of fish antihypertensive peptides distributed from 200 to 7000D, and the content of peptides from 200 to 2000D accounted for 81.7% of total peptides. The solubility at pH 2 － 12 was over 98% and its moistureabsorption was similar with soybean peptides while the rate of absorption moisture was lower than urea and higher than glycerol.

Key words: fish antihypertensive peptides, macroporous adsorption resin, ACE inhibitory activity, desalting, physico-chemical properties

中图分类号:

TS201.1

陈季旺1，孙勤1，夏文水1,2. 鱼降压肽的大孔吸附树脂脱盐及理化性质[J]. 食品科学, 2009, 30(24): 158-162.

CHEN Ji-wang1，SUN Qin1，XIA Wen-shui1,2. Desalting and Physico-chemical Properties of Antihypertensive Peptides from Grass Carp Protein with Macroporous Adsorption Resin[J]. FOOD SCIENCE, 2009, 30(24): 158-162.

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鱼降压肽的大孔吸附树脂脱盐及理化性质

Desalting and Physico-chemical Properties of Antihypertensive Peptides from Grass Carp Protein with Macroporous Adsorption Resin

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