食品科学 ›› 2009, Vol. 30 ›› Issue (24): 227-231.doi: 10.7506/spkx1002-6630-200924048

• 工艺技术 • 上一篇    下一篇

大豆发芽富集γ-氨基丁酸的工艺优化

毛 健1,2,马海乐1,*   

  1. 1.江苏大学食品与生物工程学院 2.江南大学食品学院
  • 收稿日期:2009-10-21 修回日期:2009-09-28 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 马海乐1,* E-mail:mhl@ujs.edu.cn

Optimization of Technological Conditions on γ-Aminobutyric Accumulation in Germinated Soybean

MAO Jian1,2,MA Hai-le1,*   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Received:2009-10-21 Revised:2009-09-28 Online:2009-12-15 Published:2010-12-29
  • Contact: MA Hai-le1,* E-mail:mhl@ujs.edu.cn

摘要:

以浸泡时间、发芽时间、发芽温度、谷氨酸钠(MSG)质量浓度为考察因素,γ- 氨基丁酸(GABA)含量为指标,在单因素试验的基础上,进行四因素三水平的Box-Benhnken 中心组合设计,并采用响应面分析法优化大豆发芽富集工艺。得出最佳工艺条件为浸泡时间16h、发芽温度27.5℃、发芽时间48h、MSG 质量浓度2.5mg/L。在此条件下,GABA 含量达到211.67mg/100g 干基。

关键词: 大豆, 发芽, γ-氨基丁酸, 响应面, 工艺优化

Abstract:

The effects of soak time, germination time, germination temperature and MSG on the accumulation of γ - aminobutyric acid (GABA) in germinated soybean were studied using single factor test. Subsequently, a central composite design (CCD) involving 27 tests of four variables (i.e., soak time, germinated temperature, germinated time and MSG) at three levels combined with response surface methodology were employed to optimize the γ -aminobutyric formation. The results showed that the optimum germination conditions were as follows: soak time 16 h, germination temperature 27.5 ℃, germination time 48 h and concentration of MSG solution 2.5 mg/L.Under such conditions, the content of GABA was 211.67 mg/100 g dry weight.

Key words: soybean, germination, γ-aminobutyric acid, response surface methodology, optimization of technological condition

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