食品科学 ›› 2009, Vol. 30 ›› Issue (24): 324-326.doi: 10.7506/spkx1002-6630-200924071

• 分析检测 • 上一篇    下一篇

柱前衍生-HPLC分析天山花楸多糖中的单糖组成

孟 磊,常军民* ,孙 莲,薛照芸   

  1. 新疆医科大学药学院
  • 收稿日期:2008-12-17 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 常军民 E-mail:cjmcjn2471@yahoo.com.cn
  • 基金资助:

    新疆维吾尔自治区高校科研计划重点项目(XJEDU2005119)

Use of Pre-column Derivatization HPLC for Determination of Monosaccharide Compositions and Contents in Sorbus tianschanica Ruper. Polysaccharide

MENG Lei,CHANG Jun-min*,SUN Lian,XUE Zhao-yun   

  1. College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China
  • Received:2008-12-17 Online:2009-12-15 Published:2010-12-29
  • Contact: CHANG Jun-min*, E-mail:cjmcjn2471@yahoo.com.cn

摘要:

目的:测定分析天山花楸中的单糖组成。方法:天山花楸多糖样品用2mol/L 硫酸溶液水解成单糖,用1- 苯基-3- 甲基-5- 吡唑啉酮(PMP)衍生化,采用反相高效液相色谱法于波长245nm 处检测各单糖。结果:天山花楸多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7 种单糖组成。结论:此法简单、快速、重现性好,可用于天山花楸多糖中单糖组成分析。

关键词: 高效液相色谱法, 天山花楸多糖, 柱前衍生化, 单糖

Abstract:

Objective: To determine the monosaccharide compositions in polysaccharide from Sorbus tianschanica Ruper.. Methods: Sorbus tianschanica Ruper. polysaccharide samples were hydrolyzed with 2 mol/L sulfuric acid solution, and derivatized with 1- phenyl-3 - methyl -5 - pyrazolone (PMP), and further separated by RP-HPLC using a equivalent elution process detected at 245 nm. Results: Polysaccharide of Sorbus tianschanica Ruper. was composed of mannose, rhamnose, galacturonic acid, glucuronide acid, glucose, galactose, arabinose. Conclusion: The method was simple, fast and reproducible, and can be used in determination of polysaccharide compositions of Sorbus tianschanica Ruper..

Key words: HPLC, Sorbus tianschanica Ruper. polysaccharides, pre-column derivatization, monosaccharide

中图分类号: