食品科学 ›› 2009, Vol. 30 ›› Issue (24): 359-361.doi: 10.7506/spkx1002-6630-200924080

• 分析检测 • 上一篇    下一篇

气相色谱法测定核桃等干果中的α-亚麻酸

岳巧云,张 静,刘恭源,张朋杰,杨 芳,李 蓉,郑雷青   

  1. 中山出入境检验检疫局  
  • 收稿日期:2008-12-31 修回日期:2009-07-06 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 岳巧云 E-mail:yueqy@zs.gdciq.gov.cn,qy_yue@yahoo.com.cn

GC Determination of α-Linolenate in Nuts

YUE Qiao-yun,ZHANG Jing,LIU Gong-yuan,ZHANG Peng-jie,YANG Fang,LI Rong,ZHENG Lei-qing   

  1. Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China
  • Received:2008-12-31 Revised:2009-07-06 Online:2009-12-15 Published:2010-12-29
  • Contact: YUE Qiao-yun, E-mail:yueqy@zs.gdciq.gov.cn,qy_yue@yahoo.com.cn

摘要:

采用强极性HP-Wax(30m × 0.25mm,0.5μm)色谱柱,以外标法测定核桃等干果中α - 亚麻酸含量。正己烷抽提其中的α - 亚麻酸,经过氢氧化钾- 甲醇皂化甲酯化后形成α - 亚麻酸甲酯,通过检测α - 亚麻酸甲酯的含量直接得出α - 亚麻酸的含量。α - 亚麻酸甲酯的线性检测范围为0.02~10.00mg/ml,r=0.99924,方法的回收率为95.4%~102.3%。

关键词: ?α-亚麻酸, ?α-亚麻酸甲酯, 核桃, 干果, 气相色谱法

Abstract:

α-Linolenate was extracted with n-hexane from nuts, and methylified with 2 mol/L KOH-methnoal solution. Then content of α-methyl linolenate, which could be translated to the content of α-linolenate, was quantified with gas chromatography using HP-Wax column (30 m×0.25 mm, 0.5 μm). The retention time for α-methyl linolenate was 6.325 min. This method was appicable in a linear range of 0.02-10.00 mg/ml with the correlation coefficient at 0.99924 and the average recovery for α - linolenate at 95.4%- 102.3%.

Key words: &alpha, -linolenate, &alpha, -methyl linolenate, nuts, gas chromatography

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