食品科学 ›› 2009, Vol. 30 ›› Issue (23): 196-199.doi: 10.7506/spkx1002-6300-200923044

• 基础研究 • 上一篇    下一篇

橙皮苷和绿原酸的体内外抗氧化效应研究

刘 英1,2,王之盛1,3,*,周安国1,3,蔡景义1,3   

  1. 1.四川农业大学动物营养研究所 2.成都市产品质量监督检验院 3.四川农业大学 动物抗病营养教育部重点实验室
  • 收稿日期:2008-12-09 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 王之盛 E-mail:zengyong800608@163.com
  • 基金资助:

    教育部“长江学者和创新团队发展计划”项目(IRT0555)

in vivo and in vitro Antioxidant Effects of Hesperidin and Chlorogenic Acid

LIU Ying1,2,WANG Zhi-sheng1,3,*,ZHOU An-guo1,3,CAI Jing-yi1,3   

  1. 1. Animal Nutrition Institute, Sichuan Agricultural University, Ya'an 625014, China ;2. Chengdu Product Quality Supervision and
    Inspection Institute, Chengdu 610041, China;3. Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education,
    Sichuan Agricultural University, Ya'an 625014, China
  • Received:2008-12-09 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Zhi-sheng E-mail:zengyong800608@163.com

摘要:

目的:考察橙皮苷和绿原酸的体内外抗氧化效应。方法:体外实验建立氧自由基模型,采用邻苯三酚自氧化法,产生超氧阴离子;采用H2O2/Fe2+ 体系,通过Fenton 反应产生羟自由基。测定指标为氧自由基清除率和IC50(半数抑制浓度)。体内实验以28 日龄断奶仔猪为动物模型,在基础日粮中添加橙皮苷(300mg/kg)、绿原酸(300mg/kg),进行为期4 周的饲养实验。实验结束测定仔猪血浆中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-PX)活性、过氧化氢酶(CAT)活性、抑制羟自由基能力、抗超氧阴离子能力和丙二醛(MDA)含量。结果:体外实验表明橙皮苷和绿原酸对氧自由基均有抑制作用,存在剂量效应关系,且绿原酸的效果优于橙皮苷;绿原酸显著提高仔猪血浆中谷胱甘肽过氧化物酶(GSH-Px) 、过氧化氢酶(CAT)活性以及抑制羟自由基能力(P < 0.05);橙皮苷组显著降低血浆丙二醛(MDA)含量(P < 0.05)。结论:橙皮苷和绿原酸均具有显著的体内外抗氧化效应,绿原酸的抗氧化效果优于橙皮苷。

关键词: 橙皮苷, 绿原酸, 氧自由基

Abstract:

The aim of this study was to investigate the in vitro and in vivo antioxidant effects of hesperidin and chlorogenic acid by pyrogallol autoxidation and fenton reaction and animal studies. Piglets weaned at 14-28 days were fed basal diet of cornsoybean meal as a control and experiment diets containing 300 mg/kg of hesperidin and 300 mg/kg of chlorogenic acid, respectively for four weeks. The results showed that hesperidin and chlorogenic acid were able to scavenge the oxygen free radicals in vitro in a dose-response manner and chlorogenic acid displayed higher antioxidant activity than hesperidin. Animal studies suggested that chlorogenic acid significantly enhanced the levels of serum CAT, GSH-Px and the CIHR (P<0.05) while hesperidin was able to reduce the contents of MDA (P < 0.05).

Key words: hesperidin, chlorogenic acid, oxygen free radicals

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