食品科学 ›› 2009, Vol. 30 ›› Issue (23): 110-113.doi: 10.7506/spkx1002-6300-200923023

• 基础研究 • 上一篇    下一篇

荔枝核活性成分分析及其提取物抗氧化性能研究

陆志科,黎 深   

  1. 广西大学林学院
  • 收稿日期:2008-12-31 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 陆志科 E-mail:lukz586@163.com
  • 基金资助:

    广西大学基金项目(DD180011)

Antioxidant Capacity and Preliminary Active Components Study of Litchi Seed Extract

LU Zhi-ke,LI Shen   

  1. College of Forestry ,Guangxi University, Nanning 530005, China
  • Received:2008-12-31 Online:2009-12-01 Published:2010-12-29
  • Contact: LU Zhi-ke E-mail:lukz586@163.com

摘要:

对不同荔枝核样品采用香草醛- 高氯酸比色法测定荔枝核总皂甙含量,用硝酸铝- 亚硝酸钠比色法测定总黄酮含量,用蒽酮- 硫酸法测定多糖成分的含量;结果表明:不同荔枝核样品中总皂甙含量在1.30%~1.40%之间,总黄酮含量在5.78%~6.80% 之间;多糖2.85%~3.34% 之间。以芦丁为对照,从对不同体系产生的超氧阴离子自由基、羟自由基和DPPH 自由基的清除能力及脂质过氧化的抑制活性等方面研究荔枝核提取物的抗氧化活性。结果显示:荔枝核提取物质量浓度为0.8mg/ml 时,对超氧阴离子自由基的清除率达92.96%,超过同质量浓度的芦丁;质量浓度为0.5mg/ml 时,对羟自由基的清除率达85. 56%,略低于相应对照。荔枝核提取物对脂质过氧化的抑制率随浓度的增加而增大,质量浓度为0.5mg/ml 时,抑制率达52.56%,稍低于相应对照。妃子笑荔枝核提取物质量浓度为1mg/ml 时,其清除DPPH 自由基的能力超过同质量浓度的芦丁。

关键词: 荔枝核, 提取物, 活性成分, 抗氧化活性

Abstract:

Total saponins, flavonoids and polysaccharides in litchi seed extract (LSE) of different cultivars were determined using vanillin-perchloric acid method, aluminum nitrate-sodium nitrite method and anthrone-sulfuric acid method, respectively, with their contents varying 1.30% - 1.40%, 5.78% - 6.80%, 2.85% - 3.34%, respectively. Superoxide anion radical, hydroxyl radical and DPPH free radical scavenging abilities of LSE were evaluated and compared with that of rutin. Result show that superoxide anion scavenging rate was up to 92.96% (LSE concentraction 0.8mg/ml), higher than that of rutin of the same concentration, and hydroxyl radical scavenging rate was 85.56% (LSE concentraction 0.5mg/ml), and DPPH free radical scavenging rate was 52.56% (LSE concentration 0.5mg/ml). LSE inhibited lipid peroxidation with the inhibition rate up to 52.56% (LSE concentration 0.5mg/ml).

Key words: litchi seeds, extracts, active components, antioxidant capacity

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