食品科学 ›› 2009, Vol. 30 ›› Issue (23): 270-273.doi: 10.7506/spkx1002-6300-200923061

• 生物工程 • 上一篇    下一篇

中国猴头菇栽培菌株的系统进化研究

王 磊,宿红艳* ,滕长英,韩立亚,辛成齐   

  1. 鲁东大学生命科学学院
  • 收稿日期:2008-12-01 修回日期:2009-07-27 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 王磊 E-mail:wanglei9909@163.com
  • 基金资助:

    国家自然科学基金项目(30740017);鲁东大学创新团队建设项目

Phylogenetic Study on Hericium erinaceus Cultivars in China

WANG Lei,SU Hong-yan*,TENG Chang-ying,HAN Li-ya,XIN Cheng-qi   

  1. College of Life Science, Ludong University, Yantai 264025, China
  • Received:2008-12-01 Revised:2009-07-27 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Lei E-mail:wanglei9909@163.com

摘要:

利用随机扩增多态性DNA(RAPD)标记对7 个猴头菇之间的亲缘关系进行研究,获得了猴头菇不同菌株的DNA 指纹图谱。结果显示:14 个随机引物中有3 个随机引物的扩增产物的DNA 条带表现出明显的多态性。这3个引物共扩增出44 条带,其中36 条为多态性条带,多态性位点比率为81.81%。同时利用NTSYSpc 2.1 生物软件分析7 个供试菌株之间的遗传相似性,并绘制系统进化树。

关键词: 猴头菇, RAPD, 系统进化分析

Abstract:

The phylogenetic relationship of 7 Hericium erinaceuss strains was studied using randomly amplified polymorphic DNA (RAPD) markers. The DNA fingerprints of different strains showed that there was genetic diversity among the tested strains. The results indicated that 3 out of 14 random primers were polymorphic. These 3 random primers amplified 44 bands in total, 36 bands of which showed polymorphism and the polymorphic rate was 81.81%. Furthermore, the NTSYSpc 2.1 biology software was used to analyze the genetic similarity of the tested strains and to construct the phylogenetic tree. This study can provide the molecular evidences for variety identification and phylogenetic analysis of the Hericium erinaceus.

Key words: Hericium erinaceus, RAPD, phylogenetic analysis

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