食品科学 ›› 2009, Vol. 30 ›› Issue (23): 335-339.doi: 10.7506/spkx1002-6300-200923076

• 生物工程 • 上一篇    下一篇

响应面法优化黑曲霉产纤维素酶发酵条件

冯培勇,赵彦宏,张 丽   

  1. 鲁东大学生命科学学院
  • 收稿日期:2008-12-09 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 冯培勇 E-mail:fengpeiyong2004@yahoo.com.cn
  • 基金资助:

    鲁东大学校基金项目(20053305)

Optimization of Cellulase Production by Aspergillus niger Using Response Surface Methodology

FENG Pei-yong,ZHAO Yan-hong,ZHANG Li   

  1. College of Life Science, Ludong University, Yantai 264025, China
  • Received:2008-12-09 Online:2009-12-01 Published:2010-12-29
  • Contact: FENG Pei-yong E-mail:fengpeiyong2004@yahoo.com.cn

摘要:

利用响应面法对黑曲霉产纤维素酶的发酵条件进行优化。首先通过二水平设计的Plackett-Burman 试验分析7 种因素对黑曲霉产纤维素酶活力的影响,确定发酵温度、发酵时间、装液量为影响酶活的重要因素。然后通过响应面分析得到最优条件:发酵温度31.02℃、发酵时间73.17h、装液量100.4ml。考虑实验的实际情况,确定最优条件为发酵温度31℃、发酵时间73h、装液量100ml。优化后纤维素酶活由267.81U/ml 提高到360.02U/ml,提高34.4%。

关键词: 黑曲霉, 纤维素酶, 响应面, SAS

Abstract:

Response Surface Methodology was applied to optimize the fermentation of Aspergillus niger for cellulose. In the first step, two level factorial design of Plackett-Burman was used to evaluate the influence of seven related factors. The results showed that the effects of temperature, time and packing volume on the activities of cellulase were significant among the seven factors. In the follow step, the optimal conditions of the three factors were assessed by the RSA. The ptimal conditions of the three factors were as follows: fermentation temperature of 31.02 ℃, 73.17 h of fermentation and 100.4 ml of packing volume. Under the optimal condition, the enzyme activity was increased by 34.4 %, from 267.81 to 360.02 U/ml.

Key words: Aspergillus niger, cellulase, response surface, SAS system

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