食品科学 ›› 2009, Vol. 30 ›› Issue (23): 352-355.doi: 10.7506/spkx1002-6300-200923080

• 生物工程 • 上一篇    下一篇

乳酸链球菌素对乳酸菌抑菌作用的研究

贺 松,龚芳红,张德纯* ,郭亚楠   

  1. 重庆医科大学分子医学与肿瘤研究中心,病原生物学教研室
  • 收稿日期:2008-12-19 修回日期:2009-07-25 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 张德纯 E-mail:zhangdechun46@sohu.com

Antimicrobial Activity of Nisin against Lactic Acid Bacteria

HE Song,GONG Fang-hong,ZHANG De-chun*,GUO Ya-nan   

  1. Department of Pathogenic Biology, Molecular Medicine and Tumor Research Center, Chongqing Medical University, Chongqing
    400016, China
  • Received:2008-12-19 Revised:2009-07-25 Online:2009-12-01 Published:2010-12-29
  • Contact: ZHANG De-chun E-mail:zhangdechun46@sohu.com

摘要:

目的:为了观察Nisin 对乳酸菌的抑菌效果,探讨Nisin 作为食品级载体筛选方法的最佳抑菌浓度和持续时间。方法:采用试管稀释法和纸片扩散法检测Nisin 对乳酸菌的抑菌浓度,并根据乳酸菌的OD600nm 作出生长曲线,以研究Nisin 对乳酸菌的抑菌效果。结果:Nisin 对乳品工业常用乳酸菌具有较强的抑菌作用,仅某些乳酸乳球菌对Nisin 表现出抗性;Nisin 对乳酸菌有较持久的抑菌效果,可达30~48h 以上。结论:该实验为Nisin 作为食品级载体筛选方法的最佳抑菌浓度和持续时间提供了依据。

关键词: 乳酸链球菌素, 乳酸菌, 抑菌作用, 食品级载体

Abstract:

Nisin is a bacteriocin used against food spoilage bacteria. The effect of these important antibacterial agents on the growth of lactic acid bacteria was investigated. nisin MIC values were measured by test-tube dilution and disc diffusion methods. The growth curve of lactic acid bacteria was obtained based on its OD600nm absorptions. The results showed that nisin possessed strong and persistent antimicrobial effect on common lactic acid bacteria in dairy industry except for some Lactococcus lactis strains. This study provided the theoretical basis for investigating the MIC and inhibiting time of Nisin to select food-grade recombinant Lactobacillus.

Key words: nisin, lactic acid bacterium, antimicrobial activity, food-grade vector

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